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Literature summary for 1.14.13.200 extracted from

  • Shen, B.; Hutchinson, C.R.
    Triple hydroxylation of tetracenomycin A2 to tetracenomycin C in Streptomyces glaucescens. Overexpression of the tcmG gene in Streptomyces lividans and characterization of the tetracenomycin A2 oxygenase (1994), J. Biol. Chem., 269, 30726-30733.
    View publication on PubMed
Search for more literature for 1.14.13.200

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Streptomyces lividans Streptomyces glaucescens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0018
-
 tetracenomycin A2 pH 9.0, 25°C Streptomyces glaucescens
0.082
-
 NADH pH 9.0, 25°C Streptomyces glaucescens
0.26
-
 NADPH pH 9.0, 25°C Streptomyces glaucescens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
 1 * 60000, SDS-PAGE Streptomyces glaucescens
61000
-
 gel filtration Streptomyces glaucescens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
tetracenomycin A2 + 2 O2 + 2 NAD(P)H + 2 H+ Streptomyces glaucescens biosynthesis tetracenomycin C, a polyketide antitumor antibiotic tetracenomycin C + 2 NAD(P)+ + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Streptomyces glaucescens Q7M0K6
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Streptomyces glaucescens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00429
-
 pH 9.0, 25°C Streptomyces glaucescens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
tetracenomycin A2 + 2 O2 + 2 NAD(P)H + 2 H+ biosynthesis tetracenomycin C, a polyketide antitumor antibiotic Streptomyces glaucescens tetracenomycin C + 2 NAD(P)+ + H2O
-
 ?
tetracenomycin A2 + 2 O2 + 2 NADH + 2 H+ tetracenomycin A2 i.e. methyl 10,12-dihydroxy-3,8-dimethoxy-1-methyl-6,11-dioxo-6,11-dihydrotetracene-2-carboxylate. The enzyme prefers NADPH over NADH as an electron donor Streptomyces glaucescens tetracenomycin C + 2 NAD+ + H2O tetracenomycin C = methyl (6aR,7S,10aR)-6a,7,10a,12-tetrahydroxy-3,8-dimethoxy-1-methyl-6,10,11-trioxo-6,6a,7,10,10a,11-hexahydrotetracene-2-carboxylate ?
tetracenomycin A2 + 2 O2 + 2 NADPH + 2 H+ tetracenomycin A2 i.e. methyl 10,12-dihydroxy-3,8-dimethoxy-1-methyl-6,11-dioxo-6,11-dihydrotetracene-2-carboxylate. The enzyme prefers NADPH over NADH as an electron donor Streptomyces glaucescens tetracenomycin C + 2 NADP+ + H2O tetracenomycin C = methyl (6aR,7S,10aR)-6a,7,10a,12-tetrahydroxy-3,8-dimethoxy-1-methyl-6,10,11-trioxo-6,6a,7,10,10a,11-hexahydrotetracene-2-carboxylate ?

Subunits

Subunits Comment Organism
monomer 1 * 60000, SDS-PAGE Streptomyces glaucescens

Synonyms

Synonyms Comment Organism
ElmG
-
 Streptomyces glaucescens
Tcm A2 oxygenase
-
 Streptomyces glaucescens
TcmG
-
 Streptomyces glaucescens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9 9.5 in 50 mM Tris-HC1 or 50 nm ethanolamine-HCl buffer Streptomyces glaucescens

pH Range

pH Minimum pH Maximum Comment Organism
7.5 10 pH 7.5: about 45% of maximal activity, pH 10: about 25% of maximal activity Streptomyces glaucescens

Cofactor

Cofactor Comment Organism Structure
FAD contains 1 mol of non-covalently bound FAD. The apoenzyme can be partially reconstituted in vitro by addition of FAD Streptomyces glaucescens