Activating Compound | Comment | Organism | Structure |
---|---|---|---|
7-Chloro-8-demethyl-FAD | reactivates apoenzyme | Acinetobacter sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5,5'-dithiobis(2-nitrobenzoate) | - |
Acinetobacter sp. | |
5-deaza-FAD | competitive | Acinetobacter sp. | |
p-hydroxymercuribenzoate | - |
Acinetobacter sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.004 | - |
cyclohexanone | - |
Acinetobacter sp. | |
0.02 | - |
NADPH | - |
Acinetobacter sp. | |
3.6 | - |
Cyclopentanone | - |
Acinetobacter sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cyclohexanone + NADPH + O2 | Acinetobacter sp. | enzyme plays an important role in degradation of keto-compounds by microorganisms | 1-oxa-2-oxocycloheptane + NADP+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acinetobacter sp. | - |
NCIB 9871 | - |
Purification (Comment) | Organism |
---|---|
- |
Acinetobacter sp. |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
cyclohexanone + NADPH + H+ + O2 = hexano-6-lactone + NADP+ + H2O | ter-ter mechanism | Acinetobacter sp. | |
cyclohexanone + NADPH + H+ + O2 = hexano-6-lactone + NADP+ + H2O | a stable 4a-hydroperoxy-flavin carries out a nucleophilic attack on the ketone | Acinetobacter sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.1 | - |
- |
Acinetobacter sp. |
Storage Stability | Organism |
---|---|
-20°C, more than 1 year | Acinetobacter sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(+)-camphor + NADPH + O2 | - |
Acinetobacter sp. | ? | - |
? | |
2-methylcyclohexanone + NADPH + O2 | - |
Acinetobacter sp. | 1-oxa-2-oxo-3-methylcycloheptane + NADP+ + H2O | - |
? | |
4-methylcyclohexanone + NADPH + O2 | - |
Acinetobacter sp. | 1-oxa-2-oxo-5-methylcycloheptane + NADP+ + H2O | - |
? | |
cyclobutanone + NADPH + O2 | - |
Acinetobacter sp. | 1-oxa-2-oxo-cyclopentane + NADP+ + H2O | - |
? | |
cycloheptanone + NADPH + O2 | - |
Acinetobacter sp. | 1-oxa-2-oxo-cyclooctane + NADP+ + H2O | - |
? | |
cyclohexanone + NADPH + O2 | - |
Acinetobacter sp. | 6-hexanolide + NADP+ + H2O | product: epsilon-caprolactone i.e. 1-oxa-2-oxocycloheptane | ? | |
cyclohexanone + NADPH + O2 | enzyme plays an important role in degradation of keto-compounds by microorganisms | Acinetobacter sp. | 1-oxa-2-oxocycloheptane + NADP+ + H2O | - |
? | |
cyclooctanone + NADPH + O2 | - |
Acinetobacter sp. | 1-oxa-2-oxo-cyclononane + NADP+ + H2O | - |
? | |
cyclopentanone + NADPH + O2 | - |
Acinetobacter sp. | 1-oxa-2-oxo-cyclohexane + NADP+ + H2O | - |
? | |
dihydrocarvone + NADPH + O2 | - |
Acinetobacter sp. | ? | - |
? | |
additional information | absolutely specific for NADPH as electron donor | Acinetobacter sp. | ? | - |
? | |
norcamphor + NADPH + O2 | - |
Acinetobacter sp. | ? | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Acinetobacter sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
6-Methyl-FAD | reactivates apoenzyme | Acinetobacter sp. | |
9-Aza-FAD | reactivates apoenzyme | Acinetobacter sp. | |
FAD | - |
Acinetobacter sp. | |
NADPH | electron donor | Acinetobacter sp. | |
NADPH | absolutely specific for NADPH as electron donor | Acinetobacter sp. |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.062 | - |
5-deaza-FAD | - |
Acinetobacter sp. |