Application | Comment | Organism |
---|---|---|
synthesis | chiral catalysis for the laboratory-scale transformation of racemic and prochiral ketones to chiral lactones and organic sulfur compounds to optically active sulfoxides, as a whole cell preparation and as an isolated immobilized enzyme | Acinetobacter calcoaceticus |
Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli | Acinetobacter calcoaceticus |
General Stability | Organism |
---|---|
immobilization stabilizes | Acinetobacter calcoaceticus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
bicyclo[3.2.0]hept-2-en-6-one + NADPH + O2 | Acinetobacter calcoaceticus | racemic substrate | ? | - |
? | |
bicyclo[3.2.0]hept-2-en-6-one + NADPH + O2 | Acinetobacter calcoaceticus | recombinant in E. coli | ? | - |
? | |
methyl phenyl sulfide + NADPH + H+ + O2 | Acinetobacter calcoaceticus | recombinant in E. coli | (R)-methyl phenyl sulfoxide + NADP+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acinetobacter calcoaceticus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partially, recombinant from Escherichia coli | Acinetobacter calcoaceticus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
cyclohexanone + NADPH + H+ + O2 = hexano-6-lactone + NADP+ + H2O | enantioselectivity | Acinetobacter calcoaceticus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
activity is dependent on substrate concentration and buffer conditions | Acinetobacter calcoaceticus |
Storage Stability | Organism |
---|---|
25°C, 1 M sodium sulfate, half-life 1 week | Acinetobacter calcoaceticus |
25°C, half-life 1 day | Acinetobacter calcoaceticus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
bicyclo[3.2.0]hept-2-en-6-one + NADPH + O2 | racemic substrate | Acinetobacter calcoaceticus | ? | - |
? | |
bicyclo[3.2.0]hept-2-en-6-one + NADPH + O2 | recombinant from E. coli | Acinetobacter calcoaceticus | ? | - |
? | |
bicyclo[3.2.0]hept-2-en-6-one + NADPH + O2 | recombinant in E. coli | Acinetobacter calcoaceticus | ? | - |
? | |
methyl phenyl sulfide + NADPH + H+ + O2 | recombinant from E. coli | Acinetobacter calcoaceticus | (R)-methyl phenyl sulfoxide + NADP+ + H2O | - |
? | |
methyl phenyl sulfide + NADPH + H+ + O2 | recombinant in E. coli | Acinetobacter calcoaceticus | (R)-methyl phenyl sulfoxide + NADP+ + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CHMO | - |
Acinetobacter calcoaceticus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Acinetobacter calcoaceticus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Acinetobacter calcoaceticus |