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Literature summary for 1.14.13.23 extracted from
- m-Hydroxybenzoate 4-hydroxylase from Aspergillus niger: purification and properties (1973), Indian J. Biochem. Biophys., 10, 184-190.
General Stability
| General Stability | Organism |
|---|---|
| 2-mercaptoethanol stabilizes | Aspergillus niger |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| diethyldithiocarbamate | - |
Aspergillus niger |  |
| heavy metal ions | - |
Aspergillus niger | |
| m-Aminobenzoate | - |
Aspergillus niger | |
| N-ethylmaleimide | - |
Aspergillus niger | |
| o-phenanthroline | - |
Aspergillus niger | |
| p-hydroxymercuribenzoate | - |
Aspergillus niger | |
| Salicylaldoxime | - |
Aspergillus niger | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.19 | - |
3-hydroxybenzoate | - |
Aspergillus niger | |
| 0.2 | - |
NADPH | - |
Aspergillus niger | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-hydroxybenzoate + [reduced NADPH-hemoprotein reductase] + O2 | Aspergillus niger | - |
3,4-dihydroxybenzoate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus niger | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Aspergillus niger |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| mycelium | - |
Aspergillus niger | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-hydroxybenzoate + NADPH + H+ + O2 | - |
Aspergillus niger | 3,4-dihydroxybenzoate + NADP+ + H2O | - |
r | |
| 3-hydroxybenzoate + [reduced NADPH-hemoprotein reductase] + O2 | - |
Aspergillus niger | 3,4-dihydroxybenzoate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Aspergillus niger |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | - |
- |
Aspergillus niger |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | flavoprotein | Aspergillus niger | |
| NADPH | - |
Aspergillus niger | |
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Release 2023.1 (January 2023)
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