Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.14.13.24 extracted from

  • Rajasekharan, S.; Rajasekharan, R.; Vaidyanathan, C.S.
    Substrate-mediated purification and characterization of a 3-hydroxybenzoic acid-6-hydroxylase from Micrococcus (1990), Arch. Biochem. Biophys., 278, 21-25.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Cibacron blue 50% inhibition at 0.04 mM Micrococcus sp.
Cu2+ 85% inhibition at 0.01 mM, inhibition can be reversed by thiol reagents Micrococcus sp.
Hg2+ 85% inhibition at 0.01 mM, inhibition can be reversed by thiol reagents Micrococcus sp.
p-chloromercuribenzoate at low concentrations Micrococcus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.063
-
3-hydroxybenzoate cosubstrate NADH Micrococcus sp.
0.095
-
NADH
-
Micrococcus sp.

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no requirement for metal ions Micrococcus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
67000
-
SDS-PAGE Micrococcus sp.
70000
-
gel filtration Micrococcus sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3-hydroxybenzoate + NADH + O2 Micrococcus sp. key role in metabolism of aromatic compounds 2,5-dihydroxybenzoate + NAD+ + H2O
-
?

Organism

Organism UniProt Comment Textmining
Micrococcus sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
single step purification using substrate-mediated interaction of the enzyme with blue-Sepharose, homogeneity Micrococcus sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
21.4
-
-
Micrococcus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-hydroxybenzoate + NADH + O2 strictly specific for 3-hydroxybenzoate Micrococcus sp. 2,5-dihydroxybenzoate + NAD+ + H2O
-
?
3-hydroxybenzoate + NADH + O2 key role in metabolism of aromatic compounds Micrococcus sp. 2,5-dihydroxybenzoate + NAD+ + H2O
-
?
additional information not: salicylate Micrococcus sp. ?
-
?
additional information acts also on a number of analogs of 3-hydroxybenzoate substituted in the 2, 3, 5 and 6 positions Micrococcus sp. ?
-
?
additional information not: p-hydroxybenzoate Micrococcus sp. ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 67000, SDS-PAGE Micrococcus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Micrococcus sp.

Cofactor

Cofactor Comment Organism Structure
FAD flavoprotein Micrococcus sp.
FAD 1 mol FAD per mol enzyme, exogenous addition of FAD required to regenerate oxidized FAD during catalysis Micrococcus sp.
NADH
-
Micrococcus sp.
NADPH strict preference for NADH over NADPH Micrococcus sp.