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Literature summary for 1.14.13.24 extracted from

  • Montersino, S.; Orru, R.; Barendregt, A.; Westphal, A.H.; van Duijn, E.; Mattevi, A.; van Berkel, W.J.
    Crystal structure of 3-hydroxybenzoate 6-hydroxylase uncovers lipid-assisted flavoprotein strategy for regioselective aromatic hydroxylation (2013), J. Biol. Chem., 288, 26235-26245.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Top10 cells Rhodococcus jostii

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method, using 28% (w/v) PEG 4000, 0.2 M sodium acetate, and 0.1 M Tris-HCl (pH 8.5) Rhodococcus jostii

Protein Variants

Protein Variants Comment Organism
H213A inactive, but the strength of substrate binding is not affected by the mutation Rhodococcus jostii
H213S inactive Rhodococcus jostii
Q301E inactive Rhodococcus jostii
Y105F the mutant shows less affinity for the aromatic substrate and lower catalytic efficiency compared to the wild type enzyme Rhodococcus jostii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.027
-
3-hydroxybenzoate wild type enzyme, at pH 8.0 and 25°C Rhodococcus jostii
0.186
-
3-hydroxybenzoate mutant enzyme Y105F, at pH 8.0 and 25°C Rhodococcus jostii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3-hydroxybenzoate + NADH + H+ + O2 Rhodococcus jostii
-
2,5-dihydroxybenzoate + NAD+ + H2O
-
?

Organism

Organism UniProt Comment Textmining
Rhodococcus jostii Q0SFK6
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-hydroxybenzoate + NADH + H+ + O2
-
Rhodococcus jostii 2,5-dihydroxybenzoate + NAD+ + H2O
-
?

Subunits

Subunits Comment Organism
homodimer x-ray crystallography Rhodococcus jostii

Synonyms

Synonyms Comment Organism
3-hydroxybenzoate 6-hydroxylase
-
Rhodococcus jostii
3HB6H
-
Rhodococcus jostii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3 6 3-hydroxybenzoate wild type enzyme, at pH 8.0 and 25°C Rhodococcus jostii
41
-
3-hydroxybenzoate mutant enzyme Y105F, at pH 8.0 and 25°C Rhodococcus jostii

Cofactor

Cofactor Comment Organism Structure
FAD each subunit contains a tightly but non-covalently bound FAD Rhodococcus jostii
NADH
-
Rhodococcus jostii