Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Top10 cells | Rhodococcus jostii |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapor diffusion method, using 28% (w/v) PEG 4000, 0.2 M sodium acetate, and 0.1 M Tris-HCl (pH 8.5) | Rhodococcus jostii |
Protein Variants | Comment | Organism |
---|---|---|
H213A | inactive, but the strength of substrate binding is not affected by the mutation | Rhodococcus jostii |
H213S | inactive | Rhodococcus jostii |
Q301E | inactive | Rhodococcus jostii |
Y105F | the mutant shows less affinity for the aromatic substrate and lower catalytic efficiency compared to the wild type enzyme | Rhodococcus jostii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.027 | - |
3-hydroxybenzoate | wild type enzyme, at pH 8.0 and 25°C | Rhodococcus jostii | |
0.186 | - |
3-hydroxybenzoate | mutant enzyme Y105F, at pH 8.0 and 25°C | Rhodococcus jostii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-hydroxybenzoate + NADH + H+ + O2 | Rhodococcus jostii | - |
2,5-dihydroxybenzoate + NAD+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodococcus jostii | Q0SFK6 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-hydroxybenzoate + NADH + H+ + O2 | - |
Rhodococcus jostii | 2,5-dihydroxybenzoate + NAD+ + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | x-ray crystallography | Rhodococcus jostii |
Synonyms | Comment | Organism |
---|---|---|
3-hydroxybenzoate 6-hydroxylase | - |
Rhodococcus jostii |
3HB6H | - |
Rhodococcus jostii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3 | 6 | 3-hydroxybenzoate | wild type enzyme, at pH 8.0 and 25°C | Rhodococcus jostii | |
41 | - |
3-hydroxybenzoate | mutant enzyme Y105F, at pH 8.0 and 25°C | Rhodococcus jostii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | each subunit contains a tightly but non-covalently bound FAD | Rhodococcus jostii | |
NADH | - |
Rhodococcus jostii |