Literature summary for 1.14.13.25 extracted from

Fox, B.G.; Froland, W.A.; Jollie, D.R.; Lipscomb, J.D.
Methane monooxygenase from Methylosinus trichosporium OB3b (1990), Methods Enzymol., 188, 191-202.

Search for more literature for 1.14.13.25

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.025	-	methane	-	Methylosinus trichosporium
0.035	-	furan	-	Methylosinus trichosporium
0.05	-	NADH	-	Methylosinus trichosporium

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Iron	4.3 mol Fe per mol enzyme	Methylosinus trichosporium
Iron	2 mol Fe per mol enzyme	Methylosinus trichosporium

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
additional information	-	see under subunits: molecular weights of the subunits of components	Methylosinus trichosporium
additional information	-	enzyme system consists of 3 protein components A, B, C	Methylosinus trichosporium
15100	-	component B, gel filtration	Methylosinus trichosporium
22700	-	component A: 2 * 54400 alpha, 2 * 43000 beta + 2 * 22700 gamma, sedimentation velocity, SDS-PAGE, amino acid analysis	Methylosinus trichosporium
38300	38400	component C: reductase, gel filtration	Methylosinus trichosporium
43000	-	component A: 2 * 54400 alpha, 2 * 43000 beta + 2 * 22700 gamma, sedimentation velocity, SDS-PAGE, amino acid analysis	Methylosinus trichosporium
54400	-	component A: 2 * 54400 alpha, 2 * 43000 beta + 2 * 22700 gamma, sedimentation velocity, SDS-PAGE, amino acid analysis	Methylosinus trichosporium
241000	246000	protein A hydroxylase, gel filtration	Methylosinus trichosporium

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
methane + NAD(P)H + O2	Methylosinus trichosporium	-	methanol + NAD(P)+ + H2O	-	?

Organism

Organism	UniProt	Comment	Textmining
Methylosinus trichosporium	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
improved purification protocol using stabilizing agents	Methylosinus trichosporium

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
26.1	-	purified enzyme, substrate propene	Methylosinus trichosporium

Storage Stability

Storage Stability	Organism
-80°C, more than 1 year	Methylosinus trichosporium

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
furan + NAD(P)H + O2	-	Methylosinus trichosporium	?	-	?
methane + NAD(P)H + O2	-	Methylosinus trichosporium	methanol + NAD(P)+ + H2O	-	?
propene + NAD(P)H + O2	-	Methylosinus trichosporium	1,2-epoxypropane + NAD(P)+ + H2O	-	?

Subunits

Subunits	Comment	Organism
?	component A: 2 * 54400 alpha, 2 * 43000 beta + 2 * 22700 gamma, sedimentation velocity, SDS-PAGE, amino acid analysis	Methylosinus trichosporium
More	see under molecular weight for the size of the protein components	Methylosinus trichosporium
More	enzyme system consists of 3 protein components A, B, C	Methylosinus trichosporium
More	component A is a hydroxylase	Methylosinus trichosporium

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
3.7	-	methane	-	Methylosinus trichosporium
4.4	-	Propene	-	Methylosinus trichosporium
7.6	-	furan	-	Methylosinus trichosporium

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	furan, propene	Methylosinus trichosporium

Cofactor

Cofactor	Comment	Organism	Structure
FAD	protein C, reductase component: contains 1 mol FAD per mol protein	Methylosinus trichosporium
NADH	-	Methylosinus trichosporium

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Release 2023.1 (January 2023)