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Literature summary for 1.14.13.35 extracted from
- Properties of anthranilate hydroxylase (deaminating), a flavoprotein from Trichosporon cutaneum (1987), J. Biol. Chem., 262, 69-74.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2-Aminonicotinate | activates | Cutaneotrichosporon cutaneum |  |
| N-Formyl anthranilate | activates | Cutaneotrichosporon cutaneum | |
| salicylate | activates | Cutaneotrichosporon cutaneum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50000 | - |
2 * 50000, SDS-PAGE | Cutaneotrichosporon cutaneum |
| 95000 | - |
gel filtration | Cutaneotrichosporon cutaneum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cutaneotrichosporon cutaneum | - |
yeast | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| using ammonium sulfate precipitation, column chromatography on DE23-cellulose, phenyl-Sepharose and S-300 Sephacryl | Cutaneotrichosporon cutaneum |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| anthranilate + NADPH + H+ + O2 = 2,3-dihydroxybenzoate + NADP+ + NH3 | mechanism proposed involving imine formation and hydrolysis during the reaction with the flavin peroxide formed from reduced enzyme flavin and molecular oxygen | Cutaneotrichosporon cutaneum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 4.4 | - |
- |
Cutaneotrichosporon cutaneum |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, in the dark, Na+/K+ phosphate buffer, pH 7.4, 0.1 mM EDTA, stable for at least 6 months | Cutaneotrichosporon cutaneum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-hydrazinebenzoate + NADH + O2 | - |
Cutaneotrichosporon cutaneum | ? | - |
? | |
| 3-hydroxyanthranilate + NADPH + O2 | - |
Cutaneotrichosporon cutaneum | ? | - |
? | |
| 3-methylanthranilate + NADPH + O2 | - |
Cutaneotrichosporon cutaneum | ? + NADP+ + NH3 | - |
? | |
| anthranilate + NADPH + O2 | - |
Cutaneotrichosporon cutaneum | 2,3-dihydroxybenzoate + NADP+ + NH3 | 2,3-dihydroxybenzoate i.e. o-pyrocatechuate, oxygen atom at the 3-position of the product 2,3-dihydroxybenzoate originates from O2, that at the 2-position is derived from H2O | ? | |
| N,N-dimethylanthranilate + NADPH + O2 | - |
Cutaneotrichosporon cutaneum | ? | - |
? | |
| N-methylanthranilate + NADPH + O2 | - |
Cutaneotrichosporon cutaneum | 2,3-dihydroxybenzoate + NADP+ + NH3 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 50000, SDS-PAGE | Cutaneotrichosporon cutaneum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Cutaneotrichosporon cutaneum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.7 | - |
- |
Cutaneotrichosporon cutaneum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 9.8 | less than 1% of maximal activity at pH 5.5 and pH 9.8 | Cutaneotrichosporon cutaneum |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5.9 | 9.1 | 4°C, stable for at least 3 days | Cutaneotrichosporon cutaneum |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | flavoprotein | Cutaneotrichosporon cutaneum | |
| FAD | 2 mol FAD per mol enzyme | Cutaneotrichosporon cutaneum | |
| NADPH | - |
Cutaneotrichosporon cutaneum | |
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