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Literature summary for 1.14.13.35 extracted from

  • Kumar, R.P.; Sreeleela, N.S.; Rao, P.V.S.; Vaidyanathan, C.S.
    Anthranilate hydroxylase from Aspergillus niger: evidence for the participation of iron in the double hydroxylation reaction (1973), J. Bacteriol., 113, 1213-1216.
    View publication on PubMedView publication on EuropePMC
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Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline 46% inhibition at 0.5 mM , 60% inhibition at 1 mM, 80% inhibition at 3 mM, anthranilic acid protects the enzyme from inhibition Aspergillus niger
2,2'-dipyridyl 27% inhibition at 0.5 mM, 41% inhibition at 1 mM Aspergillus niger
8-hydroxyquinoline 23% inhibition at 0.5 mM, 37% inhibition at 1 mM Aspergillus niger
EDTA 7% inhibition at 1 mM Aspergillus niger
additional information reversal of 1,10-phenanthroline inhibition by ferric-EDTA, ferrous-EDTA, ferric citrate and cytochrome c Aspergillus niger
Salicylaldoxime 40% inhibition at 0.5 mM, 52% inhibition at 1 mM Aspergillus niger

Metals/Ions

Metals/Ions Comment Organism Structure
Iron the partially purified enzyme is not activated by any metal ion but a considerable decrease in anthranilate hydroxylase activity occurs when the organism is grown on a medium deprived of iron Aspergillus niger
Iron enzyme-bound iron, participation of Fe in reaction, omission of Fe in the growth medium yields inactive preparation Aspergillus niger

Organism

Organism UniProt Comment Textmining
Aspergillus niger
-
 UBC 814
-
Aspergillus niger UBC 814
-
 UBC 814
-

Purification (Commentary)

Purification (Comment) Organism
partial, using centrifugation, protamine sulfate treatment, treatment with diethylaminoethyl-cellulose and filtration through a Buchner funnel Aspergillus niger

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.28
-
 organism grown on iron-deficient medium, no addition of other constituents Aspergillus niger
2.2
-
 organism grown on iron-deficient medium, addition of 1 mM ferric citrate, preincubation time: 5 min Aspergillus niger
4.2
-
 organism grown on iron-deficient medium, addition of 1 mM ferric citrate, preincubation time: 10 min Aspergillus niger
4.6
-
 organism grown on iron-deficient medium, addition of 1 mM ferric-EDTA Aspergillus niger
4.9
-
 organism grown on iron-deficient medium, addition of 1 mM ferric citrate, preincubation time: 15, 20 or 30 min Aspergillus niger
5.6
-
 organism grown on standard iron-sufficient medium Aspergillus niger

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
anthranilate + NADPH + O2
-
 Aspergillus niger 2,3-dihydroxybenzoate + NADP+ + NH3
-
 ?
anthranilate + NADPH + O2
-
 Aspergillus niger UBC 814 2,3-dihydroxybenzoate + NADP+ + NH3
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.2
-
 assay at Aspergillus niger

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Aspergillus niger