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Literature summary for 1.14.13.39 extracted from

  • Pufahl, R.A.; Nanjappan, P.G.; Woodard, R.W.; Marletta, M.A.
    Mechanistic probes of N-hydroxylation of L-arginine by the inducible nitric oxide synthase from murine macrophages (1992), Biochemistry, 31, 6822-6828.
    View publication on PubMed
Search for more literature for 1.14.13.39

Inhibitors

Inhibitors Comment Organism Structure
Ngamma-hydroxy-Ngamma-methyl-L-arginine preincubation at 37°C leads to irreversible inactivation, substrates protect Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.028
-
 Ngamma-hydroxy-L-arginine nonlinear regression analysis Mus musculus
0.0314
-
 Ngamma-hydroxy-L-arginine Eadie-Hofstee graph Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
2',5'-ADP-agarose affinity chromatography Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
macrophage
-
 Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 L-arginine + 3 NADPH + 4 O2 + 3 H+
-
 Mus musculus 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O
-
 ?
additional information Ngamma-hydroxylation is the first step of the reaction, Ngamma-hydroxy-L-arginine being an intermediate in the L-arginine to NO pathway Mus musculus ?
-
 ?
Ngamma-hydroxy-L-arginine + NADPH + O2
-
 Mus musculus citrulline + NADP+ + NO
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Mus musculus

Cofactor

Cofactor Comment Organism Structure
5,6,7,8-tetrahydro-L-biopterin enhances initial rate of NO-formation Mus musculus
FAD tightly enzyme-bound Mus musculus
FAD no activation by the addition of exogenous FAD Mus musculus
FMN tightly enzyme-bound Mus musculus
FMN no activation by the addition of exogenous FMN Mus musculus
NADPH
-
 Mus musculus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0265
-
 Ngamma-hydroxy-Ngamma-methyl-L-arginine
-
 Mus musculus