Literature summary for 1.14.13.39 extracted from

Yui, Y.; Hattori, R.; Kosuga, K.; Eizawa, H.;Hiki, K.; Ohkawa, S.; Ohnishi, K.; Terao, S.; Kawai, C.
Calmodulin-independent nitric oxide synthase from rat polymorphonuclear neutrophils (1991), J. Biol. Chem., 266, 3369-3371.

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General Stability

General Stability	Organism
unstable during purification	Rattus norvegicus

Inhibitors

Inhibitors	Comment	Organism	Structure
additional information	the macrophage enzyme is not inhibited by calmodulin antagonists (N-4-aminobutyl-), (N-6-aminohexyl)-5-chloro-2-naphthalene sulfonamide; the macrophage enzyme is not inhibited by calmodulin antagonists (N-6-aminohexyl)-1-naphthalene sulfonamide	Rattus norvegicus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.022	-	L-arginine	-	Rattus norvegicus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytosol	-	Rattus norvegicus	5829	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	required, calmodulin independent	Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
150000	-	gel filtration	Rattus norvegicus

Organism

Organism	UniProt	Comment	Textmining
Rattus norvegicus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
2',5'-ADP-affinity and anion exchange chromatography	Rattus norvegicus

Source Tissue

Source Tissue	Comment	Organism	Textmining
macrophage	-	Rattus norvegicus	-
neutrophil	polymorphonuclear	Rattus norvegicus	-
neutrophil	peritoneal polymorphonuclear	Rattus norvegicus	-
peritoneum	-	Rattus norvegicus	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.12	-	purified enzyme	Rattus norvegicus

Storage Stability

Storage Stability	Organism
4°C, crude, stable for at least 24 h	Rattus norvegicus
4°C, purified, t1/2: 3 h at pH 7.4	Rattus norvegicus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2 L-arginine + 3 NADPH + 4 O2 + 3 H+	-	Rattus norvegicus	2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O	guanidino-nitrogen of L-arginine is oxidized to form NO and citrulline	?

Subunits

Subunits	Comment	Organism
monomer	1 * 150000, SDS-PAGE	Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Rattus norvegicus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
additional information	-	pI: 5.6	Rattus norvegicus
7	8	-	Rattus norvegicus

pH Stability

pH Stability	pH Stability Maximum	Comment	Organism
7.4	-	purified, t1/2: 3 h, 4°C	Rattus norvegicus

Cofactor

Cofactor	Comment	Organism	Structure
5,6,7,8-tetrahydro-L-biopterin	required	Rattus norvegicus
Calmodulin	rat neutrophil enzyme is calmodulin independent	Rattus norvegicus
FAD	required	Rattus norvegicus
NADPH	-	Rattus norvegicus

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Release 2023.1 (January 2023)