Protein Variants | Comment | Organism |
---|---|---|
additional information | mutant DELTA296nNOS, missing N-terminal leader sequence, no impact on enzyme structure or catalysis, mutant DELTA349nNOS, missing N-terminal leader sequence plus three core motifs, weaker dimer interaction, slowerferric heme production, 10fold poorer affinity towards L-arginine | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.8 | - |
L-arginine | pH 7.6, 10°C, wild type | Rattus norvegicus | |
5.4 | - |
L-arginine | pH 7.6, 10°C, mutant DELTA296nNOS | Rattus norvegicus | |
68.5 | - |
L-arginine | pH 7.6, 10°C, mutant DELTA349nNOS | Rattus norvegicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | ferric heme | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 L-arginine + 3 NADPH + 4 O2 + 3 H+ | - |
Rattus norvegicus | 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
nNOS | - |
Rattus norvegicus |
NOS I | - |
Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | - |
Rattus norvegicus | |
NADPH | - |
Rattus norvegicus |