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Literature summary for 1.14.13.39 extracted from

  • Panda, K.; Adak, S.; Aulak, K.S.; Santolini, J.; McDonald, J.F.; Stuehr, D.J.
    Distinct influence of N-terminal elements on neuronal nitric-oxide synthase structure and catalysis (2003), J. Biol. Chem., 278, 37122-37131.
    View publication on PubMed
Search for more literature for 1.14.13.39

Protein Variants

Protein Variants Comment Organism
additional information mutant DELTA296nNOS, missing N-terminal leader sequence, no impact on enzyme structure or catalysis, mutant DELTA349nNOS, missing N-terminal leader sequence plus three core motifs, weaker dimer interaction, slowerferric heme production, 10fold poorer affinity towards L-arginine Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.8
-
 L-arginine pH 7.6, 10°C, wild type Rattus norvegicus
5.4
-
 L-arginine pH 7.6, 10°C, mutant DELTA296nNOS Rattus norvegicus
68.5
-
 L-arginine pH 7.6, 10°C, mutant DELTA349nNOS Rattus norvegicus

Metals/Ions

Metals/Ions Comment Organism Structure
Iron ferric heme Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 L-arginine + 3 NADPH + 4 O2 + 3 H+
-
 Rattus norvegicus 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O
-
 ?

Synonyms

Synonyms Comment Organism
nNOS
-
 Rattus norvegicus
NOS I
-
 Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
heme
-
 Rattus norvegicus
NADPH
-
 Rattus norvegicus