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Literature summary for 1.14.13.39 extracted from
- PIN inhibits nitric oxide and superoxide production from purified neuronal nitric oxide synthase (2006), Biochim. Biophys. Acta, 1760, 1445-1449.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| neuronal enzyme, stable expression in HEK-293 cells | Rattus norvegicus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ngamma-nitro-L-arginine methyl ester | nearly complete inhibition at 0.5 mM | Rattus norvegicus |  |
| PIN | human protein enzyme inhibitor, recombinantly expressed in Escherichia coli, the recombinant CREB-binding protein-bound inhibitor protein is purified by calmodulin affinity and inhibits the enzyme to a high extent at 0.001 mM | Rattus norvegicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 L-arginine + 3 NADPH + 4 O2 + 3 H+ | Rattus norvegicus | - |
2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant neuronal enzyme from HEK-293 cells by 2',5'-ADP affinity chromatography to about 90% purity | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| neuron | - |
Rattus norvegicus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 L-arginine + 3 NADPH + 4 O2 + 3 H+ | - |
Rattus norvegicus | 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| neuronal nitric oxide synthase | - |
Rattus norvegicus |
| nNOS | - |
Rattus norvegicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Rattus norvegicus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | - |
Rattus norvegicus | |
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Release 2023.1 (January 2023)
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