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Literature summary for 1.14.13.39 extracted from

  • Chartier, F.J.; Couture, M.
    Substrate-specific interactions with the heme-bound oxygen molecule of nitric-oxide synthase (2007), J. Biol. Chem., 282, 20877-20886.
    View publication on PubMed
Search for more literature for 1.14.13.39

Activating Compound

Activating Compound Comment Organism Structure
tetrahydrobiopterin
-
 Staphylococcus aureus
tetrahydrobiopterin
-
 Bacillus subtilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics and kinetic modelling, stopped-flow measurements Staphylococcus aureus
additional information
-
 additional information kinetics and kinetic modelling, stopped-flow measurements Bacillus subtilis

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ a heme enzyme, frequencies of electron transfer, overview Staphylococcus aureus
Fe2+ a heme enzyme, frequencies of electron transfer, overview Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 L-arginine + 3 NADPH + 3 H+ + 4 O2 Staphylococcus aureus
-
 2 citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O
-
 ?
2 L-arginine + 3 NADPH + 3 H+ + 4 O2 Bacillus subtilis
-
 2 citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-
Staphylococcus aureus
-
-
-

Reaction

Reaction Comment Organism Reaction ID
2 L-arginine + 3 NADPH + 3 H+ + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O reaction and kinetic mechanism Staphylococcus aureus
a
2 L-arginine + 3 NADPH + 3 H+ + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O reaction and kinetic mechanism Bacillus subtilis
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 L-arginine + 2 NADPH + 2 H+ + 2 O2 first half reaction via intermediate Nomega-hydroxy-L-arginine Staphylococcus aureus 2 Nomega-hydroxy-L-arginine + 2 NADP+ + 2 H2O
-
 ?
2 L-arginine + 2 NADPH + 2 H+ + 2 O2 first half reaction via intermediate Nomega-hydroxy-L-arginine Bacillus subtilis 2 Nomega-hydroxy-L-arginine + 2 NADP+ + 2 H2O
-
 ?
2 L-arginine + 3 NADPH + 3 H+ + 4 O2
-
 Staphylococcus aureus 2 citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O
-
 ?
2 L-arginine + 3 NADPH + 3 H+ + 4 O2
-
 Bacillus subtilis 2 citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O
-
 ?
2 L-arginine + 3 NADPH + 3 H+ + 4 O2 overall reaction, two-step oxidation of L-arginine using an O2-dependent mechanism, detailed overview Staphylococcus aureus 2 citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O
-
 ?
2 L-arginine + 3 NADPH + 3 H+ + 4 O2 overall reaction, two-step oxidation of L-arginine using an O2-dependent mechanism, detailed overview Bacillus subtilis 2 citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O
-
 ?
additional information mechanisms of oxygen activation by NOSs, overview Staphylococcus aureus ?
-
 ?
additional information mechanisms of oxygen activation by NOSs, overview Bacillus subtilis ?
-
 ?
Nomega-hydroxy-L-arginine + NADPH + H+ + O2 second half reaction via intermediate Nomega-hydroxy-L-arginine Staphylococcus aureus citrulline + nitric oxide + NADP+ + H2O
-
 ?
Nomega-hydroxy-L-arginine + NADPH + H+ + O2 second half reaction via intermediate Nomega-hydroxy-L-arginine Bacillus subtilis citrulline + nitric oxide + NADP+ + H2O
-
 ?

Synonyms

Synonyms Comment Organism
NOS
-
 Staphylococcus aureus
NOS
-
 Bacillus subtilis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Staphylococcus aureus
25
-
 assay at Bacillus subtilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
 assay at Staphylococcus aureus
7.6
-
 assay at Bacillus subtilis

Cofactor

Cofactor Comment Organism Structure
heme frequencies of electron transfer, overview Staphylococcus aureus
heme frequencies of electron transfer, overview Bacillus subtilis
NADPH
-
 Staphylococcus aureus
NADPH
-
 Bacillus subtilis