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Literature summary for 1.14.13.39 extracted from

  • Wang, Z.; Lawson, R.J.; Buddha, M.R.; Wei, C.; Crane, B.R.; Munro, A.W.; Stuehr, D.J.
    Bacterial flavodoxins support nitric oxide production by Bacillus subtilis nitric-oxide synthase (2007), J. Biol. Chem., 282, 2196-2202.
    View publication on PubMed
Search for more literature for 1.14.13.39

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli strain BL21(DE3) Bacillus subtilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics of heme-NO complex formation in the stopped-flow reaction Bacillus subtilis

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ a heme enzyme Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 L-arginine + 3 NADPH + 3 H+ + 4 O2 Bacillus subtilis
-
 2 citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Bacillus subtilis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 enzyme activities with different flavodoxins, overview Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 L-arginine + 2 NADPH + 2 H+ + 2 O2 first half reaction via intermediate Nomega-hydroxy-L-arginine Bacillus subtilis 2 Nomega-hydroxy-L-arginine + 2 NADP+ + 2 H2O
-
 ?
2 L-arginine + 3 NADPH + 3 H+ + 4 O2
-
 Bacillus subtilis 2 citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O
-
 ?
2 L-arginine + 3 NADPH + 3 H+ + 4 O2 overall reaction Bacillus subtilis 2 citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O
-
 ?
additional information the bacterial NOS enzymes have no attached flavoprotein domain to reduce their heme and so must rely on unknown bacterial proteins for electrons Bacillus subtilis ?
-
 ?
Nomega-hydroxy-L-arginine + NADPH + H+ + O2 second half reaction via intermediate Nomega-hydroxy-L-arginine Bacillus subtilis citrulline + nitric oxide + NADP+ + H2O
-
 ?

Synonyms

Synonyms Comment Organism
bacterial nitric-oxide synthase
-
 Bacillus subtilis
bNOS
-
 Bacillus subtilis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Bacillus subtilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
 assay at Bacillus subtilis

Cofactor

Cofactor Comment Organism Structure
flavodoxin reduced YkuN and YkuP containing FMN, YkuN is more efficient in supporting bsNOS catalysis, Km for YkuN is 0.0016 mM, for YkuP 0.022 mM, overview Bacillus subtilis
heme
-
 Bacillus subtilis
NADPH
-
 Bacillus subtilis