KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics of cytcochrome c reduction and of reaction with FMN and cytochrome c, overview, eNOS and nNOS differ slightly in their NADP(H) interaction and flavin thermodynamics, and show distinct electron transfer activities, two-state conformational equilibrium of the FMN subdomain, overview | Bos taurus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 L-arginine + 3 NADPH + 3 H+ + 4 O2 | Bos taurus | - |
2 citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
isozymes eNOS and nNOS | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
endothelium | - |
Bos taurus | - |
neuron | - |
Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
low eNOS activity might involve an altered interaction with NADP(H) | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 L-arginine + 3 NADPH + 3 H+ + 4 O2 | - |
Bos taurus | 2 citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O | - |
? | |
2 L-arginine + 3 NADPH + 3 H+ + 4 O2 | the electron transfer between cofactors FMN and FAD is reversible | Bos taurus | 2 citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O | - |
? | |
additional information | endothelial NOS has a 6fold lower NO synthesis activity and 6-16fold lower cytochrome c reductase activity than neuronal NOS due to a significantly different electron transfer capacities, substrate specificity and mechanism, oveview | Bos taurus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
endothelial nitric-oxide synthase | - |
Bos taurus |
endothelial NOS | - |
Bos taurus |
eNOS | - |
Bos taurus |
neuronal nitric-oxide synthase | - |
Bos taurus |
neuronal NOS | - |
Bos taurus |
nNOS | - |
Bos taurus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 25 | assay at | Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
assay at | Bos taurus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome c | - |
Bos taurus | |
FAD | - |
Bos taurus | |
FMN | - |
Bos taurus | |
NADP+ | binding mechanism | Bos taurus | |
NADPH | binding mechanism | Bos taurus |