Cloned (Comment) | Organism |
---|---|
the oxygenase domain of iNOS with a C-terminal His6-tag is expressed in Escherichia coli | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
carbon monoxide | carbon monoxide down-regulates iNOS activity by reducing its expression level or by inhibiting its activity by converting it to an inactive P420 form, the presence of dithiothreitol, L-Arg, or H4B partially inhibits the iNOSP450 to iNOSP420 conversion, whereas the presence of both L-Arg and 5,6,7,8-tetrahydro-L-biopterin completely prevents the transition | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | Q06518 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography, gel filtration | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 L-arginine + 3 NADPH + 3 H+ + 4 O2 | - |
Rattus norvegicus | 2 citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
inducible nitric oxide synthase | - |
Rattus norvegicus |
iNOS | - |
Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
5,6,7,8-tetrahydro-L-biopterin | - |
Rattus norvegicus | |
Calmodulin | - |
Rattus norvegicus | |
NADPH | - |
Rattus norvegicus |