Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | interleukin-1 induces the degradation of isozyme iNOS, iNOS protein levels in osteoarthritic chondrocytes decreases to 45.4% of the control upon treatment with interleukin-1 for 15 min, and are further reduced to 41.5% when the treatment period is extended to 2 h; tissue necrosis factor-alpha induces nNOS disappearance, interleukin-1 induces the degradation of nNOS | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Homo sapiens | 5737 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
15000 | - |
x * 15000, SDS-PAGE | Homo sapiens |
150000 | - |
SDS-PAGE | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P29475 | - |
- |
Homo sapiens | P35228 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
chondrocyte | iNOS is more frequently expressed in osteoarthritic than in normal chondrocytes, iNOS expression and activity in osteoarthritic chondrocytes is sub-maximal | Homo sapiens | - |
chondrocyte | nNOS is more frequently expressed in normal than in osteoarthritic chondrocytes | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 L-arginine + 3 NADPH + 3 H+ + 4 O2 | - |
Homo sapiens | 2 citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 15000, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
inducible NO synthase | - |
Homo sapiens |
iNOS | isoform | Homo sapiens |
neuronal NO synthase | - |
Homo sapiens |
nNOS | isoform | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Homo sapiens |
Organism | Comment | Expression |
---|---|---|
Homo sapiens | neither interleukin-1 nor tissue necrosis factor-alpha is capable of inducing nNOS synthesis for up to 48 h treatment, treatment with insulin-like growth factor-1 or transforming growth factor-beta for up to 48 h has no effect on the levels of nNOS | additional information |
Homo sapiens | treatment with insulin-like growth factor-1 or transforming growth factor-beta for up to 48 h has no effect on the levels of iNOS | additional information |
Homo sapiens | treatment with interleukin-1 or tissue necrosis factor-alpha for 16 h significantly increases iNOS levels to 429.4% of the control | up |