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Literature summary for 1.14.13.4 extracted from

  • Levy, C.C.; Frost, P.
    The metabolism of coumarin by a microorganism. V. Melilotate hydroxylase (1966), J. Biol. Chem., 241, 997-1003.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
2,2'-dipyridyl
-
Arthrobacter sp.
FeSO4
-
Arthrobacter sp.
KCN
-
Arthrobacter sp.
additional information not inhibitory: iodoacetic acid, iodoacetamide Arthrobacter sp.
N-ethylmaleimide
-
Arthrobacter sp.
p-chloromercuribenzoate
-
Arthrobacter sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.086
-
melilotate
-
Arthrobacter sp.
0.091
-
NADH
-
Arthrobacter sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3-(2-hydroxyphenyl)propanoate + NADH + O2 Arthrobacter sp. i.e. melilotate, role in metabolism of coumarin 3-(2,3-dihydroxyphenyl)propanoate + NAD+ + H2O
-
ir

Organism

Organism UniProt Comment Textmining
Arthrobacter sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Arthrobacter sp.

Renatured (Commentary)

Renatured (Comment) Organism
activity can be restored with FAD Arthrobacter sp.

Storage Stability

Storage Stability Organism
-20°C, stable for 1 week Arthrobacter sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-(2-hydroxyphenyl)propanoate + NADH + O2 i.e. melilotate, reversibility could not be demonstrated Arthrobacter sp. 3-(2,3-dihydroxyphenyl)propanoate + NAD+ + H2O reversibility could not be demonstrated ir
3-(2-hydroxyphenyl)propanoate + NADH + O2 i.e. melilotate, role in metabolism of coumarin Arthrobacter sp. 3-(2,3-dihydroxyphenyl)propanoate + NAD+ + H2O
-
ir
additional information high specificity, low activity with 3-hydroxyphenylpropanoate with 20% of melilotate reduction, phenylpropanoate with 1-2% of meliotate reduction Arthrobacter sp. ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Arthrobacter sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 7.5
-
Arthrobacter sp.

pH Range

pH Minimum pH Maximum Comment Organism
5 9 at pH 5.0 and 9.0: about 30% of maximum activity Arthrobacter sp.

Cofactor

Cofactor Comment Organism Structure
FAD flavoprotein Arthrobacter sp.
NADH
-
Arthrobacter sp.