Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,2'-dipyridyl | - |
Arthrobacter sp. | |
FeSO4 | - |
Arthrobacter sp. | |
KCN | - |
Arthrobacter sp. | |
additional information | not inhibitory: iodoacetic acid, iodoacetamide | Arthrobacter sp. | |
N-ethylmaleimide | - |
Arthrobacter sp. | |
p-chloromercuribenzoate | - |
Arthrobacter sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.086 | - |
melilotate | - |
Arthrobacter sp. | |
0.091 | - |
NADH | - |
Arthrobacter sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-(2-hydroxyphenyl)propanoate + NADH + O2 | Arthrobacter sp. | i.e. melilotate, role in metabolism of coumarin | 3-(2,3-dihydroxyphenyl)propanoate + NAD+ + H2O | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arthrobacter sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Arthrobacter sp. |
Renatured (Comment) | Organism |
---|---|
activity can be restored with FAD | Arthrobacter sp. |
Storage Stability | Organism |
---|---|
-20°C, stable for 1 week | Arthrobacter sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-(2-hydroxyphenyl)propanoate + NADH + O2 | i.e. melilotate, reversibility could not be demonstrated | Arthrobacter sp. | 3-(2,3-dihydroxyphenyl)propanoate + NAD+ + H2O | reversibility could not be demonstrated | ir | |
3-(2-hydroxyphenyl)propanoate + NADH + O2 | i.e. melilotate, role in metabolism of coumarin | Arthrobacter sp. | 3-(2,3-dihydroxyphenyl)propanoate + NAD+ + H2O | - |
ir | |
additional information | high specificity, low activity with 3-hydroxyphenylpropanoate with 20% of melilotate reduction, phenylpropanoate with 1-2% of meliotate reduction | Arthrobacter sp. | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Arthrobacter sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 7.5 | - |
Arthrobacter sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | at pH 5.0 and 9.0: about 30% of maximum activity | Arthrobacter sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | flavoprotein | Arthrobacter sp. | |
NADH | - |
Arthrobacter sp. |