Protein Variants | Comment | Organism |
---|---|---|
I244V | mutant enzyme has a 30% higher specific activity with 2-sec-butylphenol, guaiacol, and 2-hydroxybiphenyl. The Km-value for guaiacol decreases with this mutant, but the Km-value for 2-hydroxybiphenyl increase | Pseudomonas nitroreducens |
V368A/L417F | double replacement improves the efficiency of substrate hydroxylation by reducing the uncoupled oxidation of NADH. With guaiacol as substrate, the Vmax is increased and the Km-value is decreased. With 2-tert-butylphenol as substrate the turnover number is increased more than 5fold | Pseudomonas nitroreducens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Km-values for wild-type and mutant enzymes | Pseudomonas nitroreducens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas nitroreducens | - |
HBP1 | - |
Pseudomonas nitroreducens HBP1 | - |
HBP1 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-hydroxybiphenyl + NADH + O2 | - |
Pseudomonas nitroreducens | 2,3-dihydroxybiphenyl + NAD+ + H2O | - |
? | |
2-hydroxybiphenyl + NADH + O2 | - |
Pseudomonas nitroreducens HBP1 | 2,3-dihydroxybiphenyl + NAD+ + H2O | - |
? | |
2-sec-butylphenol + NADH + O2 | - |
Pseudomonas nitroreducens | 2-sec-butylcatechol + NAD+ + H2O | - |
? | |
2-sec-butylphenol + NADH + O2 | - |
Pseudomonas nitroreducens HBP1 | 2-sec-butylcatechol + NAD+ + H2O | - |
? | |
2-tert-butylphenol + NADH + O2 | - |
Pseudomonas nitroreducens | 1,2-dihydroxy-3-tert-butylbenzene + NAD+ + H2O | - |
? | |
2-tert-butylphenol + NADH + O2 | - |
Pseudomonas nitroreducens HBP1 | 1,2-dihydroxy-3-tert-butylbenzene + NAD+ + H2O | - |
? | |
guaiacol + NADH + O2 | i.e. 2-methoxyphenol | Pseudomonas nitroreducens | 2,3-dihydroxy-methoxybenzene + NAD+ + H2O | - |
? | |
guaiacol + NADH + O2 | i.e. 2-methoxyphenol | Pseudomonas nitroreducens HBP1 | 2,3-dihydroxy-methoxybenzene + NAD+ + H2O | - |
? | |
additional information | Ile244 is located in the substrate binding pocket and is involved in accomodating the phenyl substituent of the phenol | Pseudomonas nitroreducens | ? | - |
? | |
additional information | Ile244 is located in the substrate binding pocket and is involved in accomodating the phenyl substituent of the phenol | Pseudomonas nitroreducens HBP1 | ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | turnover-numbers for mutant enzymes | Pseudomonas nitroreducens | |
0.95 | - |
guaiacol | - |
Pseudomonas nitroreducens | |
11.9 | - |
2-Hydroxybiphenyl | wild-type enzyme | Pseudomonas nitroreducens | |
14.5 | - |
2-sec-Butylphenol | wild-type enzyme | Pseudomonas nitroreducens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | flavoenzyme | Pseudomonas nitroreducens | |
NADH | - |
Pseudomonas nitroreducens |