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Literature summary for 1.14.13.44 extracted from

  • Meyer, A.; Wursten, M.; Schmid, A.; Kohler, H.P.; Witholt, B.
    Hydroxylation of indole by laboratory-evolved 2-hydroxybiphenyl 3-monooxygenase (2002), J. Biol. Chem., 277, 34161-34167.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
additional information direct enzyme evolution of EC 1.14.13.44 results in an enzyme variant HbpAind, that hydroxylates indole and indole derivatives such as hydroxyindoles and 5-bromoindole. The wild-type protein does not catalyze theses reactions. HbpAind contains amino acid substitutions D222V and V368A. The activity for indole hydroxylation is increased 18fold in this variant Pseudomonas nitroreducens

Organism

Organism UniProt Comment Textmining
Pseudomonas nitroreducens
-
HBP1
-
Pseudomonas nitroreducens HBP1
-
HBP1
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-hydroxybiphenyl + NADH + O2 the activity of the mutant enzyme HbpAind is six times lower than that of the wild-type enzyme Pseudomonas nitroreducens 2,3-dihydroxybiphenyl + NAD+ + H2O
-
?
2-hydroxybiphenyl + NADH + O2 the activity of the mutant enzyme HbpAind is six times lower than that of the wild-type enzyme Pseudomonas nitroreducens HBP1 2,3-dihydroxybiphenyl + NAD+ + H2O
-
?
indole + NADH + O2
-
Pseudomonas nitroreducens ?
-
?
indole + NADH + O2
-
Pseudomonas nitroreducens HBP1 ?
-
?
additional information Asp222 is involved in substrate activation in HbpA Pseudomonas nitroreducens ?
-
?
additional information Asp222 is involved in substrate activation in HbpA Pseudomonas nitroreducens HBP1 ?
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.005
-
indole wild-type enzyme Pseudomonas nitroreducens
0.09
-
indole mutant enzyme HbpAind Pseudomonas nitroreducens
2.3
-
2-Hydroxybiphenyl mutant enzyme HbpAind Pseudomonas nitroreducens
15.6
-
2-Hydroxybiphenyl wild-type enzyme Pseudomonas nitroreducens

Cofactor

Cofactor Comment Organism Structure
NADH
-
Pseudomonas nitroreducens