Protein Variants | Comment | Organism |
---|---|---|
additional information | direct enzyme evolution of EC 1.14.13.44 results in an enzyme variant HbpAind, that hydroxylates indole and indole derivatives such as hydroxyindoles and 5-bromoindole. The wild-type protein does not catalyze theses reactions. HbpAind contains amino acid substitutions D222V and V368A. The activity for indole hydroxylation is increased 18fold in this variant | Pseudomonas nitroreducens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas nitroreducens | - |
HBP1 | - |
Pseudomonas nitroreducens HBP1 | - |
HBP1 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-hydroxybiphenyl + NADH + O2 | the activity of the mutant enzyme HbpAind is six times lower than that of the wild-type enzyme | Pseudomonas nitroreducens | 2,3-dihydroxybiphenyl + NAD+ + H2O | - |
? | |
2-hydroxybiphenyl + NADH + O2 | the activity of the mutant enzyme HbpAind is six times lower than that of the wild-type enzyme | Pseudomonas nitroreducens HBP1 | 2,3-dihydroxybiphenyl + NAD+ + H2O | - |
? | |
indole + NADH + O2 | - |
Pseudomonas nitroreducens | ? | - |
? | |
indole + NADH + O2 | - |
Pseudomonas nitroreducens HBP1 | ? | - |
? | |
additional information | Asp222 is involved in substrate activation in HbpA | Pseudomonas nitroreducens | ? | - |
? | |
additional information | Asp222 is involved in substrate activation in HbpA | Pseudomonas nitroreducens HBP1 | ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.005 | - |
indole | wild-type enzyme | Pseudomonas nitroreducens | |
0.09 | - |
indole | mutant enzyme HbpAind | Pseudomonas nitroreducens | |
2.3 | - |
2-Hydroxybiphenyl | mutant enzyme HbpAind | Pseudomonas nitroreducens | |
15.6 | - |
2-Hydroxybiphenyl | wild-type enzyme | Pseudomonas nitroreducens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Pseudomonas nitroreducens |