KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0019 | - |
2-Hydroxybiphenyl | - |
Pseudomonas nitroreducens | |
0.0097 | - |
NADH | reaction with 2-hydroxybiphenyl | Pseudomonas nitroreducens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas nitroreducens | - |
HBP1 | - |
Pseudomonas nitroreducens HBP1 | - |
HBP1 | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Pseudomonas nitroreducens |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
2-hydroxybiphenyl + NADH + H+ + O2 = 2,3-dihydroxybiphenyl + NAD+ + H2O | ternary complex mechanism | Pseudomonas nitroreducens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-hydroxybiphenyl + NADH + O2 | - |
Pseudomonas nitroreducens | 2,3-dihydroxybiphenyl + NAD+ + H2O | - |
? | |
2-hydroxybiphenyl + NADH + O2 | ternary complex mechanism in which the aromatic substrate has strict control in both the reductive and oxidative half-reaction in a way that reactions leading to substrate hydroxylation are favored over those leading to the futile formation of hydrogen peroxide. NAD+ release from the reduced enzyme-substrate complex is the slowest step in catalysis | Pseudomonas nitroreducens | 2,3-dihydroxybiphenyl + NAD+ + H2O | - |
? | |
2-hydroxybiphenyl + NADH + O2 | - |
Pseudomonas nitroreducens HBP1 | 2,3-dihydroxybiphenyl + NAD+ + H2O | - |
? | |
2-hydroxybiphenyl + NADH + O2 | ternary complex mechanism in which the aromatic substrate has strict control in both the reductive and oxidative half-reaction in a way that reactions leading to substrate hydroxylation are favored over those leading to the futile formation of hydrogen peroxide. NAD+ release from the reduced enzyme-substrate complex is the slowest step in catalysis | Pseudomonas nitroreducens HBP1 | 2,3-dihydroxybiphenyl + NAD+ + H2O | - |
? | |
2-propylphenol + NADH + O2 | - |
Pseudomonas nitroreducens | 1,2-dihydroxy-3-propylbenzene + NAD+ + H2O | - |
? | |
2-propylphenol + NADH + O2 | - |
Pseudomonas nitroreducens HBP1 | 1,2-dihydroxy-3-propylbenzene + NAD+ + H2O | - |
? | |
2-sec-butylphenol + NADH + O2 | - |
Pseudomonas nitroreducens | 2-sec-butylcatechol + NAD+ + H2O | - |
? | |
2-sec-butylphenol + NADH + O2 | - |
Pseudomonas nitroreducens HBP1 | 2-sec-butylcatechol + NAD+ + H2O | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.4 | - |
2-Hydroxybiphenyl | turnover rate refers to the enzyme monomer and not to the tetramer | Pseudomonas nitroreducens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | flavoenzyme | Pseudomonas nitroreducens | |
NADH | - |
Pseudomonas nitroreducens |