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Literature summary for 1.14.13.50 extracted from

  • Dai, M.; Rogers, J.B.; Warner, J.R.; Copley, S.D.
    A previously unrecognized step in pentachlorophenol degradation in Sphingobium chlorophenolicum is catalyzed by tetrachlorobenzoquinone reductase (PcpD) (2003), J. Bacteriol., 185, 302-310.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
additional information pentachlorphenol induces enzyme expression Sphingobium chlorophenolicum

Cloned(Commentary)

Cloned (Comment) Organism
gene pcpB, expression in Escherichia coli Sphingobium chlorophenolicum
gene pcpD, expression as C-terminally His12-tagged protein in Pseudomonas aeruginosa strain PAO1-Lac Sphingobium chlorophenolicum

Protein Variants

Protein Variants Comment Organism
moe construction of an allelic exchange mutant and a pcpD knockout mutant strains, the latter show reduced activity at high pentachlorophenol concentrations, not at low concentrations Sphingobium chlorophenolicum

Inhibitors

Inhibitors Comment Organism Structure
tetrachlorobenzoquinone inactivates the enzyme, feedback control Sphingobium chlorophenolicum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Sphingobium chlorophenolicum enzyme expression in response to pentachlorophenol hydroxylase PcpB ?
-
?
pentachlorophenol + NADPH + H+ + O2 Sphingobium chlorophenolicum first step of pentachlorophenol degradation pathway tetrachlorobenzoquinone + NADP+ + chloride + H2O
-
ir
tetrachlorobenzoquinone + NADPH Sphingobium chlorophenolicum second step in pentachlorophenol degradation, physiological role in association with pentachloro monooxygenase, overview tetrachlorohydroquinone + NADP+
-
?

Organism

Organism UniProt Comment Textmining
Sphingobium chlorophenolicum
-
strain RA-2, strain ATCC 39723, gene pcpB
-
Sphingobium chlorophenolicum
-
strain RA-2, strain ATCC 39723, gene pcpD immediately downstream of gene pcpB
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Escherichia coli to homogeneity Sphingobium chlorophenolicum
recombinant enzyme from Pseudomonas aeruginosa to over 95% homogeneity by nickel affinity chromatography Sphingobium chlorophenolicum

Reaction

Reaction Comment Organism Reaction ID
pentachlorophenol + NADPH + H+ + O2 = 2,3,5,6-tetrachloro-1,4-benzoquinone + NADP+ + chloride + H2O direct reduction by NADPH of the flavin at the active site without requirement of intermediate formation Sphingobium chlorophenolicum
pentachlorophenol + NADPH + H+ + O2 = 2,3,5,6-tetrachloro-1,4-benzoquinone + NADP+ + chloride + H2O tetrachlorobenzoquinone reductase PcpD reaction mechanism Sphingobium chlorophenolicum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information enzyme expression in response to pentachlorophenol hydroxylase PcpB Sphingobium chlorophenolicum ?
-
?
pentachlorophenol + NADPH + H+ + O2
-
Sphingobium chlorophenolicum tetrachlorobenzoquinone + NADP+ + chloride + H2O
-
ir
pentachlorophenol + NADPH + H+ + O2 first step of pentachlorophenol degradation pathway Sphingobium chlorophenolicum tetrachlorobenzoquinone + NADP+ + chloride + H2O
-
ir
tetrachlorobenzoquinone + NADPH
-
Sphingobium chlorophenolicum tetrachlorohydroquinone + NADP+
-
?
tetrachlorobenzoquinone + NADPH second step in pentachlorophenol degradation, physiological role in association with pentachloro monooxygenase, overview Sphingobium chlorophenolicum tetrachlorohydroquinone + NADP+
-
?

Synonyms

Synonyms Comment Organism
PCB hydroxylase
-
Sphingobium chlorophenolicum
PcpB
-
Sphingobium chlorophenolicum
PcpD
-
Sphingobium chlorophenolicum
pentachlorophenol hydroxylase
-
Sphingobium chlorophenolicum
pentachlorophenol monooxygenase
-
Sphingobium chlorophenolicum
tetrachlorobenzoquinone reductase
-
Sphingobium chlorophenolicum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Sphingobium chlorophenolicum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Sphingobium chlorophenolicum

Cofactor

Cofactor Comment Organism Structure
FAD flavin monooxygenase Sphingobium chlorophenolicum
NADPH
-
Sphingobium chlorophenolicum
NADPH dependent on Sphingobium chlorophenolicum