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Literature summary for 1.14.13.54 extracted from

  • Itagaki, E.
    Studies on steroid monooxygenase from Cylindrocarpon radicicola ATCC 11011. Oxygenative lactonization of androstenedione to testololactone (1986), J. Biochem., 99, 825-832.
    View publication on PubMed
Search for more literature for 1.14.13.54

Inhibitors

Inhibitors Comment Organism Structure
5-Pregnene-3beta,20alpha-diol lactonization of androstenedione Ilyonectria destructans
pregnenolone and other C21-20-ketosteroids which are the substrates for oxygenative esterification of the enzyme, strongly inhibit lactonization of androstenedione Ilyonectria destructans
progesterone strong, competitive inhibitor of lactonization of 17-ketosteroids Ilyonectria destructans
testosterone lactonization of androstenedione Ilyonectria destructans
Testosterone acetate lactonization of androstenedione Ilyonectria destructans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0004
-
 progesterone
-
 Ilyonectria destructans
0.002
-
 NADPH with androstenedione and O2 as cosubstrates Ilyonectria destructans
0.0043
-
 NADPH with progesterone and O2 as cosubstrates Ilyonectria destructans
0.04
-
 androstenedione
-
 Ilyonectria destructans
0.07
-
 O2 with progesterone and NADPH as cosubstrates Ilyonectria destructans

Organism

Organism UniProt Comment Textmining
Ilyonectria destructans
-
 ATTC 11011
-
Ilyonectria destructans ATTC 11011
-
 ATTC 11011
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Ilyonectria destructans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
17alpha-hydroxypregnenolone + NADPH + O2
-
 Ilyonectria destructans ?
-
 ?
17alpha-hydroxypregnenolone + NADPH + O2
-
 Ilyonectria destructans ATTC 11011 ?
-
 ?
17alpha-Hydroxyprogesterone + NADPH + O2 the 17-ketosteroid acts first with the enzyme, and then NADPH Ilyonectria destructans androstenedione + acetate + NADP+ + H2O 4-androstene-3,17-dione ?
17alpha-Hydroxyprogesterone + NADPH + O2 the 17-ketosteroid acts first with the enzyme, and then NADPH Ilyonectria destructans ATTC 11011 androstenedione + acetate + NADP+ + H2O 4-androstene-3,17-dione ?
androstenedione + NADPH + O2
-
 Ilyonectria destructans 3-oxo-13,17-secoandrost-4-eno-17,13-alpha-lactone + NADP+ + H2O i.e. testololactone ?
androstenedione + NADPH + O2
-
 Ilyonectria destructans ATTC 11011 3-oxo-13,17-secoandrost-4-eno-17,13-alpha-lactone + NADP+ + H2O i.e. testololactone ?
Pregnenolone + NADPH + O2
-
 Ilyonectria destructans ?
-
 ?
Pregnenolone + NADPH + O2
-
 Ilyonectria destructans ATTC 11011 ?
-
 ?
progesterone + NADPH + H+ + O2
-
 Ilyonectria destructans Testosterone acetate + NADP+ + H2O
-
 ?
progesterone + NADPH + H+ + O2
-
 Ilyonectria destructans ATTC 11011 Testosterone acetate + NADP+ + H2O
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
 progesterone monooxygenase activity Ilyonectria destructans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4
-
 half-life: 8 days Ilyonectria destructans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
 androstenedione monooxygenase reaction Ilyonectria destructans
7.8
-
 progesterone monooxygenase reaction Ilyonectria destructans

pH Range

pH Minimum pH Maximum Comment Organism
5.5 8 pH 5.5: about 20% of maximal activity, pH 8.0: about 40% of maximal activity, androstenedione monooxygenase activity Ilyonectria destructans