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Literature summary for 1.14.13.58 extracted from

  • Niemetz, R.; Altenschmidt, U.; Brucker, S.; Fuchs, G.
    Benzoyl-coenzyme-A 3-monooxygenase, a flavin-dependent hydroxylase. Purification, some properties and its role in aerobic benzoate oxidation via gentisate in a denitrifying bacterium (1995), Eur. J. Biochem., 227, 161-168.
    View publication on PubMed
Search for more literature for 1.14.13.58

Inhibitors

Inhibitors Comment Organism Structure
EDTA
-
 Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
63000
-
 1 * 63000, SDS-PAGE Pseudomonas sp.
65000
-
 gel filtration Pseudomonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Benzoyl-CoA + NADPH + O2 Pseudomonas sp. second enzyme in a variant of the aerobic benzoate degradation pathway ?
-
 ?
Benzoyl-CoA + NADPH + O2 Pseudomonas sp. KB740 second enzyme in a variant of the aerobic benzoate degradation pathway ?
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
-
-
Pseudomonas sp. KB740
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas sp.

Source Tissue

Source Tissue Comment Organism Textmining
culture condition:benzoate-grown cell grown aerobically Pseudomonas sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.812
-
-
 Pseudomonas sp.

Storage Stability

Storage Stability Organism
-70°C, 50% loss of activity after 1 week Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzoyl-CoA + NADPH + O2 no activity with NADH Pseudomonas sp. 3-hydroxybenzoyl-CoA + NADP+ + H2O
-
 ?
benzoyl-CoA + NADPH + O2 no activity with NADH Pseudomonas sp. KB740 3-hydroxybenzoyl-CoA + NADP+ + H2O
-
 ?
Benzoyl-CoA + NADPH + O2 second enzyme in a variant of the aerobic benzoate degradation pathway Pseudomonas sp. ?
-
 ?
Benzoyl-CoA + NADPH + O2 second enzyme in a variant of the aerobic benzoate degradation pathway Pseudomonas sp. KB740 ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 63000, SDS-PAGE Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Pseudomonas sp.

pH Range

pH Minimum pH Maximum Comment Organism
6.5 8.5 50% of maximal activity at pH 6.5 and at pH 8.5 Pseudomonas sp.

Cofactor

Cofactor Comment Organism Structure
FAD reaction is strictly dependent on the presence of a flavin nucleotide, FMN or FAD as cofactor Pseudomonas sp.
FMN reaction is strictly dependent on the presence of a flavin nucleotide, FMN or FAD as cofactor Pseudomonas sp.
NADPH required Pseudomonas sp.
NADPH no activity with NADH Pseudomonas sp.