Any feedback?
Literature summary for 1.14.13.6 extracted from
- Crystallization of orcinol hydroxylase from Pseudomonas putida (1970), FEBS Lett., 11, 189-192.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| in 2-3 days by addition of ammonium sulfate until turbidity | Pseudomonas putida |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 70000 | - |
ultracentrifugation | Pseudomonas putida |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas putida | - |
01 | - |
| Pseudomonas putida 1 | - |
01 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| using protamine sulfate treatment, column chromatography on DEAE-cellulose, Sephadex G-100, Sephadex G-75, a second DEAE-cellulose column chromatography and crystallization | Pseudomonas putida |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 12.9 | - |
- |
Pseudomonas putida |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| orcinol + NADH + O2 | - |
Pseudomonas putida | 2,3,5-trihydroxytoluene + NAD+ + H2O | - |
? |  |
| orcinol + NADH + O2 | - |
Pseudomonas putida 1 | 2,3,5-trihydroxytoluene + NAD+ + H2O | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | - |
assay at | Pseudomonas putida |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | flavoprotein | Pseudomonas putida | |
| FAD | 1 mol of FAD per mol of protein | Pseudomonas putida | |
| NADH | - |
Pseudomonas putida | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
