Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
SDS-PAGE | Rhodococcus erythropolis |
50000 | - |
gel filtration | Rhodococcus erythropolis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-Hydroxyphenylacetate + NADH + O2 | Rhodococcus erythropolis | enables growth on 3-hydroxyphenylacetate as sole carbon source | 2,5-Dihydroxyphenylacetate + NAD+ | - |
? | |
3-Hydroxyphenylacetate + NADH + O2 | Rhodococcus erythropolis S1 | enables growth on 3-hydroxyphenylacetate as sole carbon source | 2,5-Dihydroxyphenylacetate + NAD+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodococcus erythropolis | - |
highest enzyme activity when grown on 3-hydroxyphenylacetate | - |
Rhodococcus erythropolis S1 | - |
highest enzyme activity when grown on 3-hydroxyphenylacetate | - |
Purification (Comment) | Organism |
---|---|
- |
Rhodococcus erythropolis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2544 | - |
purified enzyme, pH 8.0, 30°C | Rhodococcus erythropolis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-hydroxyphenoxyacetate + NADH + O2 | 20% of the activity with 3-hydroxyphenylacetate | Rhodococcus erythropolis | ? | - |
? | |
2-hydroxyphenoxyacetate + NADH + O2 | 20% of the activity with 3-hydroxyphenylacetate | Rhodococcus erythropolis S1 | ? | - |
? | |
3-Hydroxyphenylacetate + NADH + O2 | - |
Rhodococcus erythropolis | 2,5-Dihydroxyphenylacetate + NAD+ | - |
? | |
3-Hydroxyphenylacetate + NADH + O2 | enables growth on 3-hydroxyphenylacetate as sole carbon source | Rhodococcus erythropolis | 2,5-Dihydroxyphenylacetate + NAD+ | - |
? | |
3-Hydroxyphenylacetate + NADH + O2 | - |
Rhodococcus erythropolis S1 | 2,5-Dihydroxyphenylacetate + NAD+ | - |
? | |
3-Hydroxyphenylacetate + NADH + O2 | enables growth on 3-hydroxyphenylacetate as sole carbon source | Rhodococcus erythropolis S1 | 2,5-Dihydroxyphenylacetate + NAD+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 45000, SDS-PAGE | Rhodococcus erythropolis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Rhodococcus erythropolis | |
additional information | no activity with NADPH | Rhodococcus erythropolis | |
NADH | - |
Rhodococcus erythropolis |