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Literature summary for 1.14.13.63 extracted from

  • van Berkel, W.J.H.; van den Tweel, W.J.J.
    Purification and characterisation of 3-hydroxyphenylacetate 6-hydroxylase: a novel FAD-dependent monooxygenase from a Flavobacterium species (1991), Eur. J. Biochem., 201, 585-591.
    View publication on PubMed
Search for more literature for 1.14.13.63

General Stability

General Stability Organism
3-hydroxyphenylacetate protects against thermal inactivation Flavobacterium sp.

Inhibitors

Inhibitors Comment Organism Structure
2-Chloromercuri-4-nitrophenol
-
 Flavobacterium sp.
5,5'-dithiobis(2-nitrobenzoate)
-
 Flavobacterium sp.
Cl- competitive to the aromatic substrate Flavobacterium sp.
NEM
-
 Flavobacterium sp.
p-hydroxymercuribenzoate
-
 Flavobacterium sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.079
-
 3-hydroxyphenylacetate
-
 Flavobacterium sp.
0.09
-
 3,4-Dihydroxyphenylacetate
-
 Flavobacterium sp.
0.14
-
 NADPH
-
 Flavobacterium sp.
0.15
-
 NADH
-
 Flavobacterium sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
63000
-
 2 * 63000, SDS-PAGE Flavobacterium sp.
150000 200000 gel filtration Flavobacterium sp.

Organism

Organism UniProt Comment Textmining
Flavobacterium sp.
-
-
-
Flavobacterium sp. JS-7
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Flavobacterium sp.

Source Tissue

Source Tissue Comment Organism Textmining
culture condition:phenylacetic acid-grown cell
-
 Flavobacterium sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
20.1
-
-
 Flavobacterium sp.

Storage Stability

Storage Stability Organism
-70°C, stable for at least 1 month Flavobacterium sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3,4-dihydroxyphenylacetate + NAD(P)H + O2 + H+
-
 Flavobacterium sp. 2,4,5-trihydroxyphenylacetate + NAD(P)+ + H2O
-
 ?
3,4-dihydroxyphenylacetate + NAD(P)H + O2 + H+
-
 Flavobacterium sp. JS-7 2,4,5-trihydroxyphenylacetate + NAD(P)+ + H2O
-
 ?
3,4-Dihydroxyphenylacetate + NADH + O2
-
 Flavobacterium sp. 2,4,5-Trihydroxyphenylacetate + NAD+
-
 ?
3,4-Dihydroxyphenylacetate + NADH + O2
-
 Flavobacterium sp. JS-7 2,4,5-Trihydroxyphenylacetate + NAD+
-
 ?
3-Hydroxyphenylacetate + NADH + O2 NADPH and NADH are oxidized at a comparable rate Flavobacterium sp. 2,5-Dihydroxyphenylacetate + NAD+
-
 ?
3-Hydroxyphenylacetate + NADH + O2 NADPH and NADH are oxidized at a comparable rate Flavobacterium sp. JS-7 2,5-Dihydroxyphenylacetate + NAD+
-
 ?
3-Hydroxyphenylacetate + NADPH + O2 NADPH and NADH are oxidized at a comparable rate Flavobacterium sp. 2,5-Dihydroxyphenylacetate + NADP+
-
 ?
3-Hydroxyphenylacetate + NADPH + O2 NADPH and NADH are oxidized at a comparable rate Flavobacterium sp. JS-7 2,5-Dihydroxyphenylacetate + NADP+
-
 ?
4-Hydroxyphenylacetate + NADH + O2
-
 Flavobacterium sp. 2,4-Dihydroxyphenylacetate + NAD+
-
 ?
additional information in the absence of oxygen the free enzyme is slowly reduced by NADPH or NADH Flavobacterium sp. ?
-
 ?
additional information in the absence of oxygen the free enzyme is slowly reduced by NADPH or NADH Flavobacterium sp. JS-7 ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 63000, SDS-PAGE Flavobacterium sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
15.6
-
 3,4-Dihydroxyphenylacetate
-
 Flavobacterium sp.
22.4
-
 3-hydroxyphenylacetate
-
 Flavobacterium sp.
35.8
-
 NADPH
-
 Flavobacterium sp.
36
-
 NADH
-
 Flavobacterium sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.3 8.9
-
 Flavobacterium sp.

pH Range

pH Minimum pH Maximum Comment Organism
7.3 8.9 50% of maximal activity at pH 7.3 and 8.9 Flavobacterium sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
6.5 7 37°C, stable Flavobacterium sp.
9
-
 strong inactivation above Flavobacterium sp.

Cofactor

Cofactor Comment Organism Structure
FAD contains 1 mol non-covalently bound FAD per mol of subunit Flavobacterium sp.