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Literature summary for 1.14.13.69 extracted from

  • Champreda, V.; Zhou, N.Y.; Leak, D.J.
    Heterologous expression of alkene monooxygenase components from Xanthobacter autotrophicus Py2 and reconstitution of the active complex (2004), FEMS Microbiol. Lett., 239, 309-318.
    View publication on PubMed
Search for more literature for 1.14.13.69

Cloned(Commentary)

Cloned (Comment) Organism
native and His-tagged protein expressed in Escherichia coli Rosetta(DE3)(pLysS) Xanthobacter autotrophicus

Metals/Ions

Metals/Ions Comment Organism Structure
Iron non-haem iron Xanthobacter autotrophicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
9650
-
 alpha2beta2gamma2, 2 * 58100, 2 * 38000, 2 * 9650, calculated from deduced amino acid sequences, masses in SDS-PAGE appear to be smaller (alpha and gamma subunits) or larger (beta subunit) Xanthobacter autotrophicus
38000
-
 alpha2beta2gamma2, 2 * 58100, 2 * 38000, 2 * 9650, calculated from deduced amino acid sequences, masses in SDS-PAGE appear to be smaller (alpha and gamma subunits) or larger (beta subunit) Xanthobacter autotrophicus
58100
-
 alpha2beta2gamma2, 2 * 58100, 2 * 38000, 2 * 9650, calculated from deduced amino acid sequences, masses in SDS-PAGE appear to be smaller (alpha and gamma subunits) or larger (beta subunit) Xanthobacter autotrophicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
propene + NADH + O2 Xanthobacter autotrophicus
-
 1,2-epoxypropane + NAD+ + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Xanthobacter autotrophicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant proteins from Escherichia coli Xanthobacter autotrophicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
propene + NADH + O2
-
 Xanthobacter autotrophicus 1,2-epoxypropane + NAD+ + H2O
-
 ?

Subunits

Subunits Comment Organism
hexamer alpha2beta2gamma2, 2 * 58100, 2 * 38000, 2 * 9650, calculated from deduced amino acid sequences, masses in SDS-PAGE appear to be smaller (alpha and gamma subunits) or larger (beta subunit) Xanthobacter autotrophicus

Synonyms

Synonyms Comment Organism
More part of a multicomponent enzyme complex Xanthobacter autotrophicus

Cofactor

Cofactor Comment Organism Structure
NADH
-
 Xanthobacter autotrophicus