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Literature summary for 1.14.13.7 extracted from
- Degradation of phenolic compounds by the yeast Candida tropicalis HP 15. II. Some properties of the first two enzymes of the degradation pathway (1986), J. Basic Microbiol., 26, 271-281.
General Stability
| General Stability | Organism |
|---|---|
| longer exposure to ultrasound drastically reduces enzyme activity | Candida tropicalis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0015 | - |
resorcinol | crude extract | Candida tropicalis |  |
| 0.005 | - |
phenol | crude extract | Candida tropicalis | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phenol + NADPH + O2 | Candida tropicalis | enzyme of phenol degradation pathway | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Candida tropicalis | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-aminophenol + O2 + NADPH | - |
Candida tropicalis | ? | - |
? | |
| 2-chlorophenol + O2 + NADPH | - |
Candida tropicalis | ? | - |
? | |
| 2-methyl-phenol + O2 + NADPH | i.e. o-cresol | Candida tropicalis | ? | - |
? | |
| 3-aminophenol + O2 + NADPH | - |
Candida tropicalis | ? | - |
? | |
| 3-chlorophenol + O2 + NADPH | - |
Candida tropicalis | ? | - |
? | |
| 3-methylphenol + O2 + NADPH | i.e. m-cresol | Candida tropicalis | ? | - |
? | |
| 4-aminophenol + O2 + NADPH | - |
Candida tropicalis | ? | - |
? | |
| 4-chlorocatechol + O2 + NADPH | - |
Candida tropicalis | ? | - |
? | |
| 4-chlorophenol + O2 + NADPH | - |
Candida tropicalis | ? | - |
? | |
| 4-methyl-phenol + O2 + NADPH | i.e. p-cresol | Candida tropicalis | ? | - |
? | |
| catechol + O2 + NADPH | - |
Candida tropicalis | ? | - |
? | |
| metol + O2 + NADPH | - |
Candida tropicalis | ? | - |
? | |
| additional information | broad specificity | Candida tropicalis | ? | - |
? | |
| additional information | not: salicylic acid | Candida tropicalis | ? | - |
? | |
| orcinol + O2 + NADPH | weak | Candida tropicalis | ? | - |
? | |
| phenol + NADPH + O2 | - |
Candida tropicalis | catechol + NADP+ + H2O | - |
? | |
| phenol + NADPH + O2 | enzyme of phenol degradation pathway | Candida tropicalis | ? | - |
? | |
| phloroglucinol + O2 + NADPH | - |
Candida tropicalis | ? | - |
? | |
| quinol + O2 + NADPH | - |
Candida tropicalis | 1,2,4-trihydroxybenzene + NADP+ + H2O | - |
? | |
| resorcinol + NADPH + O2 | - |
Candida tropicalis | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
enzyme from resorcinol grown cells | Candida tropicalis |
| 40 | - |
enzyme from phenol-induced cells | Candida tropicalis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 4 | - |
stable for some hours | Candida tropicalis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.6 | 8 | - |
Candida tropicalis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 8.7 | about 50% of activity maximum at pH 7.0 and 8.7 | Candida tropicalis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | activates | Candida tropicalis | |
| NADPH | - |
Candida tropicalis | |
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