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Literature summary for 1.14.13.7 extracted from

  • Neujahr, H.Y.; Gaal, A.
    Phenol hydroxylase from yeast. Purification and properties of the enzyme from Trichosporon cutaneum (1973), Eur. J. Biochem., 35, 386-400.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol 20% increase of activity at 1 mM Cutaneotrichosporon cutaneum

General Stability

General Stability Organism
chloride destabilizes Cutaneotrichosporon cutaneum
phosphate stabilizes Cutaneotrichosporon cutaneum

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline slight inhibition at 0.0005 to 0.001 mM Cutaneotrichosporon cutaneum
AgNO3 0.01 mM Cutaneotrichosporon cutaneum
ammonium sulfate
-
Cutaneotrichosporon cutaneum
ascorbate 52% inhibition at 50 mM Cutaneotrichosporon cutaneum
Cl- 90% inhibition at 0.1 M, irreversible Cutaneotrichosporon cutaneum
copper-chelating agents not Cutaneotrichosporon cutaneum
CuSO4 0.01 mM Cutaneotrichosporon cutaneum
FeSO4 0.1 mM Cutaneotrichosporon cutaneum
Guanidinium chloride 70-80% inhibition at 0.1 M Cutaneotrichosporon cutaneum
H2O2 71% inhibition at 0.1 M Cutaneotrichosporon cutaneum
HgCl2 0.01 mM Cutaneotrichosporon cutaneum
p-chloromercuribenzoate inhibition is reversed by dithiothreitol Cutaneotrichosporon cutaneum
Peroxidase
-
Cutaneotrichosporon cutaneum
SDS
-
Cutaneotrichosporon cutaneum
Sodium borohydride
-
Cutaneotrichosporon cutaneum
Sodium dithionite
-
Cutaneotrichosporon cutaneum
Triton X-100
-
Cutaneotrichosporon cutaneum
Urea 70-80% inhibition at 2 M Cutaneotrichosporon cutaneum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.018
-
phenol
-
Cutaneotrichosporon cutaneum
0.053
-
O2
-
Cutaneotrichosporon cutaneum
0.071
-
NADPH
-
Cutaneotrichosporon cutaneum

Metals/Ions

Metals/Ions Comment Organism Structure
additional information does not contain heme, non-heme iron or copper Cutaneotrichosporon cutaneum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
148000
-
gel filtration Cutaneotrichosporon cutaneum

Organism

Organism UniProt Comment Textmining
Cutaneotrichosporon cutaneum
-
yeast
-

Purification (Commentary)

Purification (Comment) Organism
homogeneity Cutaneotrichosporon cutaneum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
8.3
-
-
Cutaneotrichosporon cutaneum

Storage Stability

Storage Stability Organism
-20°C, 4-6 weeks stable Cutaneotrichosporon cutaneum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-aminophenol + O2 + NADPH
-
Cutaneotrichosporon cutaneum ?
-
?
2-chlorophenol + O2 + NADPH
-
Cutaneotrichosporon cutaneum ?
-
?
2-fluorophenol + O2 + NADPH
-
Cutaneotrichosporon cutaneum ?
-
?
3-aminophenol + O2 + NADPH
-
Cutaneotrichosporon cutaneum ?
-
?
3-chlorophenol + O2 + NADPH
-
Cutaneotrichosporon cutaneum ?
-
?
3-fluorophenol + O2 + NADPH
-
Cutaneotrichosporon cutaneum ?
-
?
4-aminophenol + O2 + NADPH
-
Cutaneotrichosporon cutaneum ?
-
?
4-chlorophenol + O2 + NADPH
-
Cutaneotrichosporon cutaneum ?
-
?
4-fluorophenol + O2 + NADPH
-
Cutaneotrichosporon cutaneum ?
-
?
additional information broad specificity, reaction results in the formation of the corresponding o-diols Cutaneotrichosporon cutaneum ?
-
?
phenol + NADPH + O2
-
Cutaneotrichosporon cutaneum catechol + NADP+ + H2O
-
?
quinol + O2 + NADPH
-
Cutaneotrichosporon cutaneum 1,2,4-trihydroxybenzene + NADP+ + H2O
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2 7.6 in phosphate buffer Cutaneotrichosporon cutaneum
8.2
-
in Tris-Cl buffer Cutaneotrichosporon cutaneum

pH Stability

pH Stability pH Stability Maximum Comment Organism
5
-
stable for at least 7 days Cutaneotrichosporon cutaneum

Cofactor

Cofactor Comment Organism Structure
FAD flavoprotein Cutaneotrichosporon cutaneum
FAD 1 mol FAD per mol of enzyme Cutaneotrichosporon cutaneum
NADPH
-
Cutaneotrichosporon cutaneum