Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | 20% increase of activity at 1 mM | Cutaneotrichosporon cutaneum |
General Stability | Organism |
---|---|
chloride destabilizes | Cutaneotrichosporon cutaneum |
phosphate stabilizes | Cutaneotrichosporon cutaneum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | slight inhibition at 0.0005 to 0.001 mM | Cutaneotrichosporon cutaneum | |
AgNO3 | 0.01 mM | Cutaneotrichosporon cutaneum | |
ammonium sulfate | - |
Cutaneotrichosporon cutaneum | |
ascorbate | 52% inhibition at 50 mM | Cutaneotrichosporon cutaneum | |
Cl- | 90% inhibition at 0.1 M, irreversible | Cutaneotrichosporon cutaneum | |
copper-chelating agents | not | Cutaneotrichosporon cutaneum | |
CuSO4 | 0.01 mM | Cutaneotrichosporon cutaneum | |
FeSO4 | 0.1 mM | Cutaneotrichosporon cutaneum | |
Guanidinium chloride | 70-80% inhibition at 0.1 M | Cutaneotrichosporon cutaneum | |
H2O2 | 71% inhibition at 0.1 M | Cutaneotrichosporon cutaneum | |
HgCl2 | 0.01 mM | Cutaneotrichosporon cutaneum | |
p-chloromercuribenzoate | inhibition is reversed by dithiothreitol | Cutaneotrichosporon cutaneum | |
Peroxidase | - |
Cutaneotrichosporon cutaneum | |
SDS | - |
Cutaneotrichosporon cutaneum | |
Sodium borohydride | - |
Cutaneotrichosporon cutaneum | |
Sodium dithionite | - |
Cutaneotrichosporon cutaneum | |
Triton X-100 | - |
Cutaneotrichosporon cutaneum | |
Urea | 70-80% inhibition at 2 M | Cutaneotrichosporon cutaneum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.018 | - |
phenol | - |
Cutaneotrichosporon cutaneum | |
0.053 | - |
O2 | - |
Cutaneotrichosporon cutaneum | |
0.071 | - |
NADPH | - |
Cutaneotrichosporon cutaneum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | does not contain heme, non-heme iron or copper | Cutaneotrichosporon cutaneum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
148000 | - |
gel filtration | Cutaneotrichosporon cutaneum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cutaneotrichosporon cutaneum | - |
yeast | - |
Purification (Comment) | Organism |
---|---|
homogeneity | Cutaneotrichosporon cutaneum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
8.3 | - |
- |
Cutaneotrichosporon cutaneum |
Storage Stability | Organism |
---|---|
-20°C, 4-6 weeks stable | Cutaneotrichosporon cutaneum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-aminophenol + O2 + NADPH | - |
Cutaneotrichosporon cutaneum | ? | - |
? | |
2-chlorophenol + O2 + NADPH | - |
Cutaneotrichosporon cutaneum | ? | - |
? | |
2-fluorophenol + O2 + NADPH | - |
Cutaneotrichosporon cutaneum | ? | - |
? | |
3-aminophenol + O2 + NADPH | - |
Cutaneotrichosporon cutaneum | ? | - |
? | |
3-chlorophenol + O2 + NADPH | - |
Cutaneotrichosporon cutaneum | ? | - |
? | |
3-fluorophenol + O2 + NADPH | - |
Cutaneotrichosporon cutaneum | ? | - |
? | |
4-aminophenol + O2 + NADPH | - |
Cutaneotrichosporon cutaneum | ? | - |
? | |
4-chlorophenol + O2 + NADPH | - |
Cutaneotrichosporon cutaneum | ? | - |
? | |
4-fluorophenol + O2 + NADPH | - |
Cutaneotrichosporon cutaneum | ? | - |
? | |
additional information | broad specificity, reaction results in the formation of the corresponding o-diols | Cutaneotrichosporon cutaneum | ? | - |
? | |
phenol + NADPH + O2 | - |
Cutaneotrichosporon cutaneum | catechol + NADP+ + H2O | - |
? | |
quinol + O2 + NADPH | - |
Cutaneotrichosporon cutaneum | 1,2,4-trihydroxybenzene + NADP+ + H2O | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | 7.6 | in phosphate buffer | Cutaneotrichosporon cutaneum |
8.2 | - |
in Tris-Cl buffer | Cutaneotrichosporon cutaneum |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | - |
stable for at least 7 days | Cutaneotrichosporon cutaneum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | flavoprotein | Cutaneotrichosporon cutaneum | |
FAD | 1 mol FAD per mol of enzyme | Cutaneotrichosporon cutaneum | |
NADPH | - |
Cutaneotrichosporon cutaneum |