Literature summary for 1.14.13.8 extracted from

Koukouritaki, S.B.; Poch, M.T.; Henderson, M.C.; Siddens, L.K.; Krueger, S.K.; VanDyke, J.E.; Williams, D.E.; Pajewski, N.M.; Wang, T.; Hines, R.N.
Identification and functional analysis of common human flavin-containing monooxygenase 3 genetic variants (2007), J. Pharmacol. Exp. Ther., 320, 266-273.

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Cloned(Commentary)

Cloned (Comment)	Organism
DNA and amino acid sequence determination and analysis of multiple samples of gene FMO3, expression of clones in Spodoptera frugiperda Sf9 insect cell microsomes via baculovirus transfection system, the FMO multigene family consists of a five-gene cluster at 1q24.3, comprising FMO1-4 and FMO6p, and a second cluster of five genes at 1q24.2, comprising FMO7p-11p, and a single gene, FMO5, at 1q21.1, encoding a total of five active proteins in humans	Homo sapiens

Cloned (Comment)

Organism

DNA and amino acid sequence determination and analysis of multiple samples of gene FMO3, expression of clones in Spodoptera frugiperda Sf9 insect cell microsomes via baculovirus transfection system, the FMO multigene family consists of a five-gene cluster at 1q24.3, comprising FMO1-4 and FMO6p, and a second cluster of five genes at 1q24.2, comprising FMO7p-11p, and a single gene, FMO5, at 1q21.1, encoding a total of five active proteins in humans

Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
E158K	naturally occuring polymorphism of FMO3, frequency in different human populations, the mutation has an impact on protein structure, overview	Homo sapiens
E24D	naturally occuring polymorphism of FMO3, frequency in different human populations, the mutation has an impact on protein structure, overview, the mutant shows altered substrate specificity compared to the wild-type mutant	Homo sapiens
E308G	naturally occuring polymorphism of FMO3, frequency in different human populations, the mutation has an impact on protein structure, overview	Homo sapiens
K416N	naturally occuring polymorphism of FMO3, frequency in different human populations, the mutation has an impact on protein structure, overview, the mutant shows altered substrate specificity compared to the wild-type mutant	Homo sapiens
additional information	determination and analysis of frequencies of 18 FMO3 single-nucleotide polymorphisms in 202 Hispanics of Mexican descent, 201 African Americans, and 200 non-Latino whites, synonymous mutations, and hypomorphic haplotypes, overview	Homo sapiens
N61K	naturally occuring polymorphism of FMO3, frequency in different human populations, the mutation has an impact on protein structure, overview, the mutant shows altered substrate specificity compared to the wild-type mutant	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism
additional information	-	additional information	kinetics of wild-type and mutant structural variants	Homo sapiens
0.0209	-	trimethylamine	recombinant wild-type enzyme, pH 8.5	Homo sapiens
0.0228	-	trimethylamine	recombinant mutant E24D, pH 8.5	Homo sapiens
0.0373	-	trimethylamine	recombinant mutant K416N, pH 8.5	Homo sapiens
0.0502	-	ethylenethiourea	recombinant mutant K416N, pH 8.5	Homo sapiens
0.0557	-	ethylenethiourea	recombinant wild-type enzyme, pH 8.5	Homo sapiens
0.0665	-	sulindac	recombinant mutant K416N, pH 8.5	Homo sapiens
0.0718	-	Methimazole	recombinant wild-type enzyme, pH 8.5	Homo sapiens
0.0761	-	ethylenethiourea	recombinant mutant E24D, pH 8.5	Homo sapiens
0.0773	-	Methimazole	recombinant mutant K416N, pH 8.5	Homo sapiens
0.0854	-	Methimazole	recombinant mutant E24D, pH 8.5	Homo sapiens
0.1208	-	sulindac	recombinant mutant E24D, pH 8.5	Homo sapiens
0.1233	-	Methimazole	recombinant mutant N61K, pH 8.5	Homo sapiens
0.1501	-	sulindac	recombinant wild-type enzyme, pH 8.5	Homo sapiens
0.2071	-	sulindac	recombinant mutant N61K, pH 8.5	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
microsome	-	Homo sapiens	-	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
additional information	Homo sapiens	the enzyme catalyzes the NADPH-dependent N-and S-oxidation of a variety of therapeutics, environmental toxicants, carcinogens, and nutrients	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	isozyme FMO3	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	-	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
ethylenethiourea + NADPH + O2	-	Homo sapiens	ethylenethiourea S-oxide + NADP+ + H2O	-	?
methimazole + NADPH + O2	-	Homo sapiens	?	-	?
additional information	the enzyme catalyzes the NADPH-dependent N-and S-oxidation of a variety of therapeutics, environmental toxicants, carcinogens, and nutrients	Homo sapiens	?	-	?
sulindac sulfide + NADPH + O2	-	Homo sapiens	(S,R)-sulindac + NADP+ + H2O	-	?
trimethylamine + NADPH + O2	-	Homo sapiens	trimethylamine N-oxide + NADP+ + H2O	-	?

Synonyms

Synonyms	Comment	Organism
FMO	-	Homo sapiens
More	the enzyme belongs to the FMO multigene family	Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism
0.003	-	sulindac	recombinant mutant N61K, pH 8.5	Homo sapiens
0.004	-	Methimazole	recombinant mutant N61K, pH 8.5	Homo sapiens
0.034	-	trimethylamine	recombinant wild-type enzyme, pH 8.5	Homo sapiens
0.036	-	ethylenethiourea	recombinant wild-type enzyme, pH 8.5	Homo sapiens
0.042	-	ethylenethiourea	recombinant mutant K416N, pH 8.5	Homo sapiens
0.044	-	sulindac	recombinant mutant E24D, pH 8.5	Homo sapiens
0.047	-	Methimazole	recombinant mutant K416N, pH 8.5	Homo sapiens
0.047	-	sulindac	recombinant wild-type enzyme, pH 8.5	Homo sapiens
0.05	-	ethylenethiourea	recombinant mutant E24D, pH 8.5	Homo sapiens
0.051	-	trimethylamine	recombinant mutant K416N, pH 8.5	Homo sapiens
0.065	-	trimethylamine	recombinant mutant E24D, pH 8.5	Homo sapiens
0.068	-	sulindac	recombinant mutant K416N, pH 8.5	Homo sapiens
0.124	-	Methimazole	recombinant wild-type enzyme, pH 8.5	Homo sapiens
0.22	-	Methimazole	recombinant mutant E24D, pH 8.5	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8.5	-	assay at	Homo sapiens

Cofactor

Cofactor	Comment	Organism	Structure
FAD	-	Homo sapiens
NADPH	-	Homo sapiens