Cloned (Comment) | Organism |
---|---|
genetic and sequence analysis | Pseudomonas denitrificans (nom. rej.) |
General Stability | Organism |
---|---|
upon handling or storing CobG preparations, the sulfur content, the A400, and the enzymatic activity drops simultaneously | Pseudomonas denitrificans (nom. rej.) |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | iron-sulfur protein | Pseudomonas denitrificans (nom. rej.) |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
precorrin-3A + NADH + H+ + O2 | Pseudomonas denitrificans (nom. rej.) | pathway to coenzyme B12, biosynthesis of the corrin macrocycle, catalyzes a complex oxidative reaction involving C20 hydroxylation and gamma-lactone formation from ring-A acetate to C1 | precorrin-3B + NAD+ + H2O | - |
ir | |
precorrin-3A + NADH + H+ + O2 | Pseudomonas denitrificans (nom. rej.) SC510 (RifT) | pathway to coenzyme B12, biosynthesis of the corrin macrocycle, catalyzes a complex oxidative reaction involving C20 hydroxylation and gamma-lactone formation from ring-A acetate to C1 | precorrin-3B + NAD+ + H2O | - |
ir | |
precorrin-3A + NADH + O2 | Pseudomonas denitrificans (nom. rej.) | - |
precorrin-3B + NAD+ + H2O | - |
? | |
precorrin-3A + NADH + O2 | Pseudomonas denitrificans (nom. rej.) SC510 (RifT) | - |
precorrin-3B + NAD+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas denitrificans (nom. rej.) | - |
- |
- |
Pseudomonas denitrificans (nom. rej.) SC510 (RifT) | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas denitrificans (nom. rej.) |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.048 | - |
- |
Pseudomonas denitrificans (nom. rej.) |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
precorrin-3A + NADH + H+ + O2 | - |
Pseudomonas denitrificans (nom. rej.) | precorrin-3B + NAD+ + H2O | - |
ir | |
precorrin-3A + NADH + H+ + O2 | pathway to coenzyme B12, biosynthesis of the corrin macrocycle, catalyzes a complex oxidative reaction involving C20 hydroxylation and gamma-lactone formation from ring-A acetate to C1 | Pseudomonas denitrificans (nom. rej.) | precorrin-3B + NAD+ + H2O | - |
ir | |
precorrin-3A + NADH + H+ + O2 | - |
Pseudomonas denitrificans (nom. rej.) SC510 (RifT) | precorrin-3B + NAD+ + H2O | - |
ir | |
precorrin-3A + NADH + H+ + O2 | pathway to coenzyme B12, biosynthesis of the corrin macrocycle, catalyzes a complex oxidative reaction involving C20 hydroxylation and gamma-lactone formation from ring-A acetate to C1 | Pseudomonas denitrificans (nom. rej.) SC510 (RifT) | precorrin-3B + NAD+ + H2O | - |
ir | |
precorrin-3A + NADH + O2 | - |
Pseudomonas denitrificans (nom. rej.) | precorrin-3B + NAD+ + H2O | - |
? | |
precorrin-3A + NADH + O2 | - |
Pseudomonas denitrificans (nom. rej.) SC510 (RifT) | precorrin-3B + NAD+ + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CobG | - |
Pseudomonas denitrificans (nom. rej.) |