Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Thermobifida fusca |
General Stability | Organism |
---|---|
PAMO that has been activated at 50°C can be stored for several days at room temperature without any loss in activity | Thermobifida fusca |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.059 | - |
phenylacetone | 30°C, pH 7.5, 0.1 mM NADPH | Thermobifida fusca | |
0.26 | - |
2-dodecanone | 30°C, pH 7.5, 0.1 mM NADPH | Thermobifida fusca | |
0.36 | - |
benzylacetone | 30°C, pH 7.5, 0.1 mM NADPH | Thermobifida fusca | |
0.83 | - |
(2-methylphenyl)acetone | 30°C, pH 7.5, 0.1 mM NADPH | Thermobifida fusca | |
0.86 | - |
methyl 4-tolylsulfide | 30°C, pH 7.5, 0.1 mM NADPH | Thermobifida fusca | |
2.2 | - |
4-hydroxyacetophenone | 30°C, pH 7.5, 0.1 mM NADPH | Thermobifida fusca | |
15 | - |
bicyclohept-2-en-6-one | 30°C, pH 7.5, 0.1 mM NADPH | Thermobifida fusca |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
65000 | - |
1 * 65000, SDS-PAGE, small amount of approx. 7% is present as a dimer | Thermobifida fusca |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermobifida fusca | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant PAMO | Thermobifida fusca |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2-methylphenyl)acetone + NADPH + O2 | - |
Thermobifida fusca | ? + NADP+ + O2 | - |
? | |
2-dodecanone + NADPH + O2 | - |
Thermobifida fusca | ? + NADP+ | - |
? | |
4-hydroxyacetophenone + NADPH + O2 | - |
Thermobifida fusca | acetic acid 4-hydroxyphenyl ester + NADP+ + O2 | - |
? | |
benzylacetone + NADPH + O2 | - |
Thermobifida fusca | ? + NADP+ + H2O | - |
? | |
bicyclohept-2-en-6-one + NADPH + O2 | - |
Thermobifida fusca | ? + NADP+ + O2 | - |
? | |
ethionamide + NADPH + O2 | - |
Thermobifida fusca | ? + NADP+ + O2 | - |
? | |
methyl 4-tolylsulfide + NADPH + O2 | - |
Thermobifida fusca | ? + NADP+ + O2 | - |
? | |
phenylacetone + NADPH + H+ + O2 | - |
Thermobifida fusca | benzyl acetate + NADP+ + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 65000, SDS-PAGE, small amount of approx. 7% is present as a dimer | Thermobifida fusca |
Synonyms | Comment | Organism |
---|---|---|
Baeyer-Villiger monooxygenase | - |
Thermobifida fusca |
BVMO | - |
Thermobifida fusca |
PAMO | - |
Thermobifida fusca |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
4fold higher activity when compared with the activity at 25°C | Thermobifida fusca |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
52 | - |
50% loss of activity after 24 h and 48 h in the absence and presence of FAD, respectively | Thermobifida fusca |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.23 | - |
2-dodecanone | 30°C, pH 7.5, 0.1 mM NADPH | Thermobifida fusca | |
0.34 | - |
4-hydroxyacetophenone | 30°C, pH 7.5, 0.1 mM NADPH | Thermobifida fusca | |
1.1 | - |
bicyclohept-2-en-6-one | 30°C, pH 7.5, 0.1 mM NADPH | Thermobifida fusca | |
1.8 | - |
benzylacetone | 30°C, pH 7.5, 0.1 mM NADPH | Thermobifida fusca | |
1.9 | - |
phenylacetone | 30°C, pH 7.5, 0.1 mM NADPH | Thermobifida fusca | |
2 | - |
(2-methylphenyl)acetone | 30°C, pH 7.5, 0.1 mM NADPH | Thermobifida fusca | |
2.1 | - |
methyl 4-tolylsulfide | 30°C, pH 7.5, 0.1 mM NADPH | Thermobifida fusca |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Thermobifida fusca |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 9 | more than 80% of maximal activity | Thermobifida fusca |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Thermobifida fusca | |
NADPH | can not be replaced by NADH | Thermobifida fusca |