Literature summary for 1.14.14.1 extracted from

Zhao, Y.; White, M.A.; Muralidhara, B.K.; Sun, L.; Halpert, J.R.; Stout, C.D.
Structure of microsomal cytochrome P450 2B4 complexed with the antifungal drug bifonazole: insight into P450 conformational plasticity and membrane interaction (2006), J. Biol. Chem., 281, 5973-5981.

Search for more literature for 1.14.14.1

Cloned(Commentary)

Cloned (Comment)	Organism
expression of mutant H226Y in Escherichia coli	Oryctolagus cuniculus

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant mutant enzyme H226Y in complex with bifonazole, X-ray diffraction structure determination and analysis at 2.3 A resolution	Oryctolagus cuniculus

Protein Variants

Protein Variants	Comment	Organism
H226Y	site-directed mutagenesis, altered coordination of the Fe2+ ion and binding of inhibitor bifonazole	Oryctolagus cuniculus

Inhibitors

Inhibitors	Comment	Organism	Structure
bifonazole	an anti-fungal drug, complex formation with CYP2B4, inhibits monooxygenase activity and induces a type II binding spectrum in CYP2B4 mutant H226Y, plastic regions efficiently, because conformational changes are clustered, the 2B4-bifonazole dimer is different from the ligand-free 2B4dH dimer, which involves the coordination of His226 to the heme iron of the other monomer	Oryctolagus cuniculus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
microsome	-	Oryctolagus cuniculus	-	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Fe2+	heme iron, His226 is involved in metal ion coordination	Oryctolagus cuniculus

Organism

Organism	UniProt	Comment	Textmining
Oryctolagus cuniculus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant mutant H226Y from Escherichia coli by ion exchange chromatography and ultracentrifugation	Oryctolagus cuniculus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
7-ethoxy-trifluoromethylcoumarin + [reduced NADPH-hemoprotein reductase] + O2	O-deethylation	Oryctolagus cuniculus	?	-	?
additional information	regiospecific and stereo-specific activity, overview	Oryctolagus cuniculus	?	-	?

Subunits

Subunits	Comment	Organism
dimer	-	Oryctolagus cuniculus

Synonyms

Synonyms	Comment	Organism
CYP2B4	-	Oryctolagus cuniculus
cytochrome P450 2B4	-	Oryctolagus cuniculus

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Release 2023.1 (January 2023)