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Literature summary for 1.14.14.11 extracted from

  • Panke, S.; Witholt, B.; Schmid, A.; Wubbolts, M.G.
    Towards a biocatalyst for (S)-styrene oxide production: characterization of the styrene degradation pathway of Pseudomonas sp. strain VLB120 (1998), Appl. Environ. Microbiol., 64, 2032-2043.
    View publication on PubMedView publication on EuropePMC
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Application

Application Comment Organism
synthesis production of optically pure (S)-styrene oxide, an important building block in organic synthesis. Recombinant Escherichia coli producing styrene monooxygenase catalyzes the formation of (S)-2-phenyloxirane from inexpensive styrene with an excellent enantiomeric excess of more than 99% at rates up to 180 U/g (dry weight) of cells Pseudomonas sp.

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pseudomonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
styrene + FADH2 + O2 Pseudomonas sp.
-
 (S)-2-phenyloxirane + FAD + H2O
-
 ?
styrene + FADH2 + O2 Pseudomonas sp. VLB120
-
 (S)-2-phenyloxirane + FAD + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
-
-
Pseudomonas sp. VLB120
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
styrene + FADH2 + O2
-
 Pseudomonas sp. (S)-2-phenyloxirane + FAD + H2O
-
 ?
styrene + FADH2 + O2 (S)-styrene oxide is produced with an enantiomeric excess of more than 99% Pseudomonas sp. (S)-2-phenyloxirane + FAD + H2O
-
 ?
styrene + FADH2 + O2
-
 Pseudomonas sp. VLB120 (S)-2-phenyloxirane + FAD + H2O
-
 ?
styrene + FADH2 + O2 (S)-styrene oxide is produced with an enantiomeric excess of more than 99% Pseudomonas sp. VLB120 (S)-2-phenyloxirane + FAD + H2O
-
 ?