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Literature summary for 1.14.14.16 extracted from
- Purification and reconstitution of the steroid 21-hydroxylase system (cytochrome P-450-linked mixed function oxidase system) of bovine adrenocortical microsomes (1981), Biochim. Biophys. Acta, 664, 33-48.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Emulgen 911 | - |
Bos taurus | |
| Emulgen 913 | maximum with 0.008% v/v | Bos taurus |  |
| lysophosphatidylcholine | - |
Bos taurus | |
| sodium cholate | - |
Bos taurus | |
| sodium deoxycholate | - |
Bos taurus | |
| Triton X-100 | - |
Bos taurus | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| antibody to cytochrome P-450BPA | - |
Bos taurus | |
| antibody to NADPH-cytochrome P-450 reductase | - |
Bos taurus | |
| carbon monoxide | - |
Bos taurus | |
| additional information | not inhibited by catalase; not inhibited by superoxide dismutase, cytochrome b5, NADH-cytochrome b5 reductase | Bos taurus | |
| Phenylisocyanide | inhibition at 0.5 mM | Bos taurus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| microsome | - |
Bos taurus | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 47500 | - |
SDS-PAGE | Bos taurus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
beef | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | 3.6% carbohydrate | Bos taurus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| enzyme system consisting of cytochrome P-450S21 and NADPH-cytochrome P-450 reductase | Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| adrenal gland | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0052 | - |
N-demethylation of (+)-benzphetamine | Bos taurus |
| 0.0077 | - |
21-hydroxylation of progesterone | Bos taurus |
| 0.0195 | - |
cytochrome P-450-linked mixed function oxidase system from adrenal gland, 21-hydroxylation of 17alpha-hydroxyprogesterone | Bos taurus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -70°C, 0.2 M potassium phospate, pH 7.4, 20% glycerol, 0.1 mM EDTA, 3 months without loss of activity | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (+)-benzphetamine + [reduced NADPH-cytochrome P-450 reductase] + O2 | - |
Bos taurus | N-demethyl-benzphetamine + [oxidized NADPH-cytochrome P-450 reductase] + H2O | - |
? | |
| 17alpha-hydroxyprogesterone + NADH + O2 | - |
Bos taurus | 17,21-dihydroxyprogesterone + NAD+ + H2O | - |
? | |
| DELTA5-pregnen-3beta-ol-20-one + NADH + O2 | no substrate of cytochrome P-450-linked mixed function oxidase system | Bos taurus | deoxycorticosterone + NAD+ + H2O | - |
? | |
| progesterone + NADH + O2 | - |
Bos taurus | deoxycorticosterone + NAD+ + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | steroid 21-hydroxylase system consists of cytochrome P-450S21, NADPH-cytochrome P-450 reductase, EC 1.6.2.4, and steroid 21-monooxygenase, EC 1.14.99.10 | Bos taurus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
- |
Bos taurus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| cytochrome P450S21 | steroid 21-hydroxylase system consists of cytochrome P-450S21, NADPH-cytochrome P-450 reductase (EC 1.6.2.4) and steroid 21-monooxygenase (EC 1.14.99.10) | Bos taurus |
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