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Literature summary for 1.14.14.17 extracted from

  • Abe, I.; Seki, T.; Umehara, K.; Miyase, T.; Noguchi, H.; Sakakibara, J.; Ono, T.
    Green tea polyphenols: novel and potent inhibitors of squalene epoxidase (2000), Biochem. Biophys. Res. Commun., 268, 767-771.
    View publication on PubMed

Application

Application Comment Organism
medicine cholesterol lowering effect of green tea may be attributed to the enzyme inhibitory activities of its gallocatechins Rattus norvegicus

Cloned(Commentary)

Cloned (Comment) Organism
-
Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
(-)-epicatechin-3-O-gallate 50% inhibition at 0.0013 mM Rattus norvegicus
(-)-epigallocatechin-3-O-gallate 50% inhibition at 0.00069 mM, noncompetitive Rattus norvegicus
(-)-gallocatechin-3-O-gallate 50% inhibition at 0.00067 mM Rattus norvegicus
epigallocatechin-3-O-gallate
-
Rattus norvegicus
additional information cholesterol lowering effect of green tea gallocatechins may be attributed to their potent squalene oxidase inhibition; not inhibitory: flavan-3-ols with galloyl group at C-3 Rattus norvegicus
theasinensin A 50% inhibition at 0.00013 mM Rattus norvegicus
trisnorsqualene alcohol 0.004 mM, 50% inhibition Sus scrofa
trisnorsqualene cyclopropylamine 0.002 mM, 50% inhibition Sus scrofa
trisnorsqualene difluoromethylidene 0.0054 mM, 50% inhibition Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0036
-
squalene
-
Rattus norvegicus
0.0038
-
squalene 37°C, pH 7.4 Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-
Sus scrofa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Rattus norvegicus

Reaction

Reaction Comment Organism Reaction ID
squalene + [reduced NADPH-hemoprotein reductase] + O2 = (3S)-2,3-epoxy-2,3-dihydrosqualene + [oxidized NADPH-hemoprotein reductase] + H2O proposed mechanism Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
squalene + electron donor + O2
-
Rattus norvegicus (S)-squalene-2,3-epoxide + oxidized electron donor + H2O
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0683
-
squalene N-terminal trucated recombinant enzyme Rattus norvegicus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00074
-
epigallocatechin-3-O-gallate non-competitive, non-time dependent inhibition Rattus norvegicus
0.00074
-
(-)-epigallocatechin-3-O-gallate 37°C, pH 7.4 Rattus norvegicus