Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | complete inhibition at 1 mM | Paenibacillus sp. | |
5,5'-dithio-bis-2-nitrobenzoic acid | 94% inhibition at 0.5 mM | Paenibacillus sp. | |
CaCl2 | 16% inhibition at 1 mM | Paenibacillus sp. | |
CoCl2 | 74% inhibition at 1 mM | Paenibacillus sp. | |
CuCl2 | complete inhibition at 1 mM | Paenibacillus sp. | |
EDTA | 68% inhibition at 1 mM | Paenibacillus sp. | |
iodoacetate | 46% inhibition at 1 mM | Paenibacillus sp. | |
MnCl2 | 66% inhibition at 1 mM | Paenibacillus sp. | |
N-ethylmaleimide | 98% inhibition at 1 mM | Paenibacillus sp. | |
p-chloromercuribenzoic acid | complete inhibition at 0.5 mM | Paenibacillus sp. | |
ZnCl2 | complete inhibition at 1 mM | Paenibacillus sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.36 | - |
benzothiophene | pH 7.0, 55°C | Paenibacillus sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43000 | - |
4 * 43000, SDS-PAGE | Paenibacillus sp. |
200000 | - |
gel filtration | Paenibacillus sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Paenibacillus sp. | Q9LBX2 | - |
- |
Paenibacillus sp. A11-2 | Q9LBX2 | - |
- |
Purification (Comment) | Organism |
---|---|
native enzyme 116fold from strain All-2 by two different steps of anion exchange chromatography and dialysis, followed by hydrophobic interaction and hydroxyapatite chromatography | Paenibacillus sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
18.6 | - |
purified enzyme, pH 7.0, 55°C | Paenibacillus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-methyldibenzothiophene + 2 FMNH2 + 2 O2 | - |
Paenibacillus sp. | 2-methyldibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O | - |
? | |
3-methylbenzothiophene + 2 FMNH2 + 2 O2 | - |
Paenibacillus sp. | 3-methylbenzothiophene-1,1-dioxide + 2 FMN + 2 H2O | - |
? | |
3-methyldibenzothiophene + 2 FMNH2 + 2 O2 | - |
Paenibacillus sp. | 3-methyldibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O | - |
? | |
4,6-diethyldibenzothiophene + 2 FMNH2 + 2 O2 | - |
Paenibacillus sp. | 4,6-diethyldibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O | - |
? | |
4,6-dimethyldibenzothiophene + 2 FMNH2 + 2 O2 | low activity | Paenibacillus sp. | 4,6-dimethyldibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O | - |
? | |
4,6-dimethyldibenzothiophene + 2 FMNH2 + 2 O2 | low activity | Paenibacillus sp. A11-2 | 4,6-dimethyldibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O | - |
? | |
4-methyldibenzothiophene + 2 FMNH2 + 2 O2 | low activity | Paenibacillus sp. | 4-methyldibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O | - |
? | |
4-methyldibenzothiophene + 2 FMNH2 + 2 O2 | low activity | Paenibacillus sp. A11-2 | 4-methyldibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O | - |
? | |
5-methylbenzothiophene + 2 FMNH2 + 2 O2 | - |
Paenibacillus sp. | 5-methylbenzothiophene-5,5-dioxide + 2 FMN + 2 H2O | - |
? | |
benzothiophene + 2 FMNH2 + 2 O2 | best substrate | Paenibacillus sp. | benzothiophene-5,5-dioxide + 2 FMN + 2 H2O | - |
? | |
benzothiophene + 2 FMNH2 + 2 O2 | best substrate | Paenibacillus sp. A11-2 | benzothiophene-5,5-dioxide + 2 FMN + 2 H2O | - |
? | |
dibenzothiophene + 2 FMNH2 + 2 O2 | low reactivity of TdsC toward dibenzothiophene at high substrate concentrations | Paenibacillus sp. | dibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O | - |
? | |
dibenzothiophene + 2 FMNH2 + 2 O2 | low reactivity of TdsC toward dibenzothiophene at high substrate concentrations | Paenibacillus sp. A11-2 | dibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O | - |
? | |
additional information | substrate specificity of enzyme TsdC with dibenzothiophene derivatives and benzothiophene derivatives, overview. No or poor activity with 4,6-dipropyldibenzothiophene, 4,6-dibutyldibenzothiophene, and 1-methyldibenzothiophene | Paenibacillus sp. | ? | - |
? | |
additional information | substrate specificity of enzyme TsdC with dibenzothiophene derivatives and benzothiophene derivatives, overview. No or poor activity with 4,6-dipropyldibenzothiophene, 4,6-dibutyldibenzothiophene, and 1-methyldibenzothiophene | Paenibacillus sp. A11-2 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 43000, SDS-PAGE | Paenibacillus sp. |
Synonyms | Comment | Organism |
---|---|---|
benzothiophene monooxygenase | - |
Paenibacillus sp. |
BT monooxygenase | - |
Paenibacillus sp. |
DBT monooxygenase | - |
Paenibacillus sp. |
dibenzothiophene monooxygenase | - |
Paenibacillus sp. |
TdsC | - |
Paenibacillus sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
- |
Paenibacillus sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 65 | activity range | Paenibacillus sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
purified enzyme, retains more than 80% of its activity after exposure for 30 min | Paenibacillus sp. |
70 | - |
purified enzyme, no activity remaining after 30 min | Paenibacillus sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Paenibacillus sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMNH2 | - |
Paenibacillus sp. | |
additional information | FAD and lumiflavin have only weak activity as reducing agents | Paenibacillus sp. | |
additional information | FMN oxidoreductase TdsD, a NADH-dependent FMN oxidoreductase, absolutely required for TdsC activity, maximum activity is obtained at a TdsD/TdsC molar ratio of 0.5, no activity in the absence of TdsD | Paenibacillus sp. | |
NADH | - |
Paenibacillus sp. | |
NADPH | can substitute for NADH to some extent | Paenibacillus sp. |