Cloned (Comment) | Organism |
---|---|
gene bdsC, DNA and amino acid sequence determination and analysis, recombinant functional expression in Escherichia coli strain JM109, coexpression with a flavin reductase Frb, encoded by gene frb, UniProt ID Q75V96. Coexpression of frb with the DBT-desulfurization genes (bdsABC) from Bacillus subtilis strain WU-S2B is critical for high DBT-desulfurizing ability over a wide temperature range of 20-55°C, coexpression screening using indigo production as selective indication | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | Q8GRC5 | gene bdsC | - |
Bacillus subtilis WU-S2B | Q8GRC5 | gene bdsC | - |
Synonyms | Comment | Organism |
---|---|---|
DBT monooxygenase | - |
Bacillus subtilis |
dibenzothiophene monooxygenase | - |
Bacillus subtilis |
General Information | Comment | Organism |
---|---|---|
physiological function | flavin reductase or flavin-dependent monooxygenase efficiently couples with the other component in two-component monooxygenases. Coexpression of frb with the DBT-desulfurization genes (bdsABC) from Bacillus subtilis strain WU-S2B is critical for high DBT-desulfurizing ability over a wide temperature range of 20-55°C | Bacillus subtilis |