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Literature summary for 1.14.14.21 extracted from
- Purification, characterization and crystallization of enzymes for dibenzothiophene desulfurization (2000), Bioseparation, 9, 185-188.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | flavin reductase (DszD) is essential for the enzyme activity of DszC | Rhodococcus erythropolis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 4-chloromercuribenzoic acid | - |
Rhodococcus erythropolis |  |
| additional information | thiol reagents and metal chelators inhibit the enzyme activity | Rhodococcus erythropolis |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | a metal ion might be involved in DszC activity | Rhodococcus erythropolis |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 45000 | - |
6 * 45000, SDS-PAGE | Rhodococcus erythropolis |
| 250000 | - |
gel filtration | Rhodococcus erythropolis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rhodococcus erythropolis | A0A0C6DRW4 | gene dszC | - |
| Rhodococcus erythropolis D-1 | A0A0C6DRW4 | gene dszC | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme 24fold to homogeneity from strain D-1 by ammonium sulfate fractionation, anion exchange chromatography, hydrophobic interaction chromatography, and another different step of anion exchange chromatography, followed by gel filtration | Rhodococcus erythropolis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 133 | - |
purified enzyme, pH 7.0, 30°C | Rhodococcus erythropolis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2,8-dimethyldibenzothiophene + 2 FMNH2 + 2 O2 | 129% of the activity with dibenzothiophene | Rhodococcus erythropolis | 2,8-dimethyldibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O | - |
? | |
| 2,8-dimethyldibenzothiophene + 2 FMNH2 + 2 O2 | 129% of the activity with dibenzothiophene | Rhodococcus erythropolis D-1 | 2,8-dimethyldibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O | - |
? | |
| 4,6-dimethyldibenzothiophene + 2 FMNH2 + 2 O2 | 52% of the activity with dibenzothiophene | Rhodococcus erythropolis | 4,6-dimethyldibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O | - |
? | |
| 4,6-dimethyldibenzothiophene + 2 FMNH2 + 2 O2 | 52% of the activity with dibenzothiophene | Rhodococcus erythropolis D-1 | 4,6-dimethyldibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O | - |
? | |
| dibenzothiophene + 2 FMNH2 + 2 O2 | - |
Rhodococcus erythropolis | dibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O | - |
? | |
| dibenzothiophene + 2 FMNH2 + 2 O2 | - |
Rhodococcus erythropolis D-1 | dibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O | - |
? | |
| additional information | no or poor activity with 3,4-benzo-dibenzothiophene, biphenyl, carbazole, dibenzofuran, and fluorene, substrate specificity, overview. Interaction with purified flavin reductase from the non-DBT-desulfurizing bacterium, Paenibacillus polymyxa A-1, with DszC | Rhodococcus erythropolis | ? | - |
? | |
| additional information | no or poor activity with 3,4-benzo-dibenzothiophene, biphenyl, carbazole, dibenzofuran, and fluorene, substrate specificity, overview. Interaction with purified flavin reductase from the non-DBT-desulfurizing bacterium, Paenibacillus polymyxa A-1, with DszC | Rhodococcus erythropolis D-1 | ? | - |
? | |
| thioxanthen-9-one + FMNH2 + O2 | 45% of the activity with dibenzothiophene | Rhodococcus erythropolis | ? | - |
? | |
| thioxanthen-9-one + FMNH2 + O2 | 45% of the activity with dibenzothiophene | Rhodococcus erythropolis D-1 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homohexamer | 6 * 45000, SDS-PAGE | Rhodococcus erythropolis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| DBT monooxygenase | - |
Rhodococcus erythropolis |
| dszC | - |
Rhodococcus erythropolis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Rhodococcus erythropolis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Rhodococcus erythropolis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FMNH2 | - |
Rhodococcus erythropolis | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| additional information | flavin reductase (DszD) is essential for the enzyme activity of DszC | Rhodococcus erythropolis |
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