Crystallization (Comment) | Organism |
---|---|
purified enzyme DszC, hanging drop vapour diffusion method, using a reservoir suliton containing 30% polyethylene glycol, 0.1 M sodium citrate, pH 5.6, and 0.2 M ammonium acetate, 4°C, X-ray diffraction structure determination and analysis | Rhodococcus erythropolis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dibenzothiophene + 2 FMNH2 + 2 O2 | Rhodococcus erythropolis | - |
dibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O | - |
? | |
dibenzothiophene + 2 FMNH2 + 2 O2 | Rhodococcus erythropolis D-1 | - |
dibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodococcus erythropolis | A0A0C6DRW4 | gene dszC | - |
Rhodococcus erythropolis D-1 | A0A0C6DRW4 | gene dszC | - |
Purification (Comment) | Organism |
---|---|
native enzyme to homogeneity by hydrophobic interaction and anion exchange chromatography. and gel filtration | Rhodococcus erythropolis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,8-dimethyldibenzothiophene + 2 FMNH2 + 2 O2 | best substrate | Rhodococcus erythropolis | 2,8-dimethyldibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O | - |
? | |
2,8-dimethyldibenzothiophene + 2 FMNH2 + 2 O2 | best substrate | Rhodococcus erythropolis D-1 | 2,8-dimethyldibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O | - |
? | |
3,4-benzo-dibenzothiophene + 2 FMNH2 + 2 O2 | low activity | Rhodococcus erythropolis | 3,4-benzo-dibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O | - |
? | |
3,4-benzo-dibenzothiophene + 2 FMNH2 + 2 O2 | low activity | Rhodococcus erythropolis D-1 | 3,4-benzo-dibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O | - |
? | |
4,6-dimethyldibenzothiophene + 2 FMNH2 + 2 O2 | - |
Rhodococcus erythropolis | 4,6-dimethyldibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O | - |
? | |
4,6-dimethyldibenzothiophene + 2 FMNH2 + 2 O2 | - |
Rhodococcus erythropolis D-1 | 4,6-dimethyldibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O | - |
? | |
dibenzothiophene + 2 FMNH2 + 2 O2 | - |
Rhodococcus erythropolis | dibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O | - |
? | |
dibenzothiophene + 2 FMNH2 + 2 O2 | high activity | Rhodococcus erythropolis | dibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O | - |
? | |
dibenzothiophene + 2 FMNH2 + 2 O2 | - |
Rhodococcus erythropolis D-1 | dibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O | - |
? | |
dibenzothiophene + 2 FMNH2 + 2 O2 | high activity | Rhodococcus erythropolis D-1 | dibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O | - |
? | |
thioxanthen-9-one + 2 FMNH2 + 2 O2 | - |
Rhodococcus erythropolis | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DBT monooxygenase | - |
Rhodococcus erythropolis |
dibenzothiophene monooxygenase | - |
Rhodococcus erythropolis |
dszC | - |
Rhodococcus erythropolis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Rhodococcus erythropolis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Rhodococcus erythropolis |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme is involved in the pathway of microbial dibenzothiophene desulfurization, overview | Rhodococcus erythropolis |