Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Geobacillus thermodenitrificans |
Crystallization (Comment) | Organism |
---|---|
crystals of LadA and the LadA:FMN complex are grown using the hanging-drop vapor-diffusion method at 18°C, crystal structure of the enzyme in the apoenzyme form and its complex with FMNH2 | Geobacillus thermodenitrificans |
Protein Variants | Comment | Organism |
---|---|---|
C14A | point mutation completely abolishes the catalytic activity | Geobacillus thermodenitrificans |
H17F | the mutant maintains the same dimeric form as the wild type, point mutation completely abolishes the catalytic activity | Geobacillus thermodenitrificans |
H311F | the mutant maintains the same dimeric form as the wild type, point mutation completely abolishes the catalytic activity | Geobacillus thermodenitrificans |
Q79L | the mutant maintains the same dimeric form as the wild type, point mutation completely abolishes the catalytic activity | Geobacillus thermodenitrificans |
T63F | the mutant maintains the same dimeric form as the wild type, point mutation completely abolishes the catalytic activity | Geobacillus thermodenitrificans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50466 | - |
2 * 50466, calculated from sequence | Geobacillus thermodenitrificans |
105000 | - |
gel filtration | Geobacillus thermodenitrificans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
a long-chain alkane + FMNH2 + O2 | Geobacillus thermodenitrificans | - |
a long-chain primary alcohol + FMN + H2O | - |
? | |
a long-chain alkane + FMNH2 + O2 | Geobacillus thermodenitrificans NG80-2 | - |
a long-chain primary alcohol + FMN + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus thermodenitrificans | A4IU28 | - |
- |
Geobacillus thermodenitrificans NG80-2 | A4IU28 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Geobacillus thermodenitrificans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
a long-chain alkane + FMNH2 + O2 | - |
Geobacillus thermodenitrificans | a long-chain primary alcohol + FMN + H2O | - |
? | |
a long-chain alkane + FMNH2 + O2 | the enzyme is capable of converting alkanes ranging from C15 to C36 into their corresponding primary alcohols | Geobacillus thermodenitrificans | a long-chain primary alcohol + FMN + H2O | - |
? | |
a long-chain alkane + FMNH2 + O2 | - |
Geobacillus thermodenitrificans NG80-2 | a long-chain primary alcohol + FMN + H2O | - |
? | |
a long-chain alkane + FMNH2 + O2 | the enzyme is capable of converting alkanes ranging from C15 to C36 into their corresponding primary alcohols | Geobacillus thermodenitrificans NG80-2 | a long-chain primary alcohol + FMN + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 50466, calculated from sequence | Geobacillus thermodenitrificans |
Synonyms | Comment | Organism |
---|---|---|
LADA | - |
Geobacillus thermodenitrificans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Geobacillus thermodenitrificans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMNH2 | - |
Geobacillus thermodenitrificans |