Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant C106A enzymes in Escherichia coli strain JM101 | Vibrio harveyi |
Protein Variants | Comment | Organism |
---|---|---|
C106A | site-directed mutagenesis, catalytic properties are similar to the wild-type enzyme, mutant shows 60% of wild-type quantum yield | Vibrio harveyi |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5-decyl-4a-hydroxy-4a,5-dihydroriboflavin-5'-phosphate | binding and fluorescence quantum yield studies of the substance as a model, complexed with the enzyme in a 1:1 molcular ratio leading to 80% and 90% inhibition of wild-type and mutant C106A at 0.01 mM, respectively, binds to the active site | Vibrio harveyi |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vibrio harveyi | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant C106A enzymes from Escherichia coli strain JM101 to over 95% purity | Vibrio harveyi |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
a long-chain aldehyde + FMNH2 + O2 = a long-chain fatty acid + FMN + H2O + hnu | reaction mechanism and scheme, cofactor binding | Vibrio harveyi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
decanal + FMNH2 + O2 | - |
Vibrio harveyi | decanoate + FMN + H2O + hn | - |
ir |
Synonyms | Comment | Organism |
---|---|---|
bacterial luciferase | - |
Vibrio harveyi |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
23 | - |
assay at | Vibrio harveyi |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Vibrio harveyi |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
4a-hydroxy-4a,5-dihydroriboflavin-5'-phosphate | model bioluminescence emitter molecule, binding and fluorescence quantum yield studies, complexed with the enzyme in a 1:1 molcular ratio | Vibrio harveyi | |
FMNH2 | physiological cofactor | Vibrio harveyi |