Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Sodium acetate | activates mutant E328A | Vibrio harveyi |
Protein Variants | Comment | Organism |
---|---|---|
A74F | site-directed mutagenesis, the mutant shows reduced activity and increased Km compared to the wild-type enzyme | Vibrio harveyi |
A74G | site-directed mutagenesis, the mutant shows increased activity compared to the wild-type enzyme | Vibrio harveyi |
E328A | site-directed mutagenesis, the mutant shows highly reduced activity and increased Km compared to the wild-type enzyme, the activity is rescued by addition of sodium acetate, but not by phosphate, at pH 6.0-8.0 with increasing activity at lower pH | Vibrio harveyi |
E328D | site-directed mutagenesis, the mutant shows highly reduced activity and increased Km compared to the wild-type enzyme | Vibrio harveyi |
E328F | site-directed mutagenesis, the mutant shows reduced activity and increased Km compared to the wild-type enzyme | Vibrio harveyi |
E328H | site-directed mutagenesis, the mutant shows highly reduced activity and increased Km compared to the wild-type enzyme | Vibrio harveyi |
E328L | site-directed mutagenesis, the mutant shows highly reduced activity and increased Km compared to the wild-type enzyme | Vibrio harveyi |
E328Q | site-directed mutagenesis, the mutant shows highly reduced activity and increased Km compared to the wild-type enzyme | Vibrio harveyi |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | stopped-flow and Michaelis-Menten kinetics of wild-type and mutant enzymes | Vibrio harveyi | |
0.0002 | - |
FMNH2 | pH 7.0, 23°C, recombinant mutants E328Q and E328H | Vibrio harveyi | |
0.0003 | - |
FMNH2 | pH 7.0, 23°C, recombinant mutants E328L and E328A | Vibrio harveyi | |
0.0004 | - |
FMNH2 | pH 7.0, 23°C, recombinant mutant E328D | Vibrio harveyi | |
0.0006 | - |
FMNH2 | pH 7.0, 23°C, recombinant wild-type enzyme | Vibrio harveyi | |
0.0015 | - |
FMNH2 | pH 7.0, 23°C, recombinant mutant A74G | Vibrio harveyi | |
0.0016 | - |
decanal | pH 7.0, 23°C, recombinant wild-type enzyme and mutant A74G | Vibrio harveyi | |
0.0023 | - |
decanal | pH 7.0, 23°C, recombinant mutant E328Q | Vibrio harveyi | |
0.0033 | - |
decanal | pH 7.0, 23°C, recombinant mutant E328A | Vibrio harveyi | |
0.0039 | - |
FMNH2 | pH 7.0, 23°C, recombinant mutant E328F | Vibrio harveyi | |
0.0045 | - |
decanal | pH 7.0, 23°C, recombinant mutant E328F | Vibrio harveyi | |
0.005 | - |
decanal | pH 7.0, 23°C, recombinant mutant E328D | Vibrio harveyi | |
0.0087 | - |
FMNH2 | pH 7.0, 23°C, recombinant mutant A74F | Vibrio harveyi | |
0.0095 | - |
decanal | pH 7.0, 23°C, recombinant mutant E328H | Vibrio harveyi | |
0.0097 | - |
decanal | pH 7.0, 23°C, recombinant mutant E328L | Vibrio harveyi | |
0.0173 | - |
decanal | pH 7.0, 23°C, recombinant mutant A74F | Vibrio harveyi |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vibrio harveyi | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
a long-chain aldehyde + FMNH2 + O2 = a long-chain fatty acid + FMN + H2O + hnu | reaction mechanism via 4a-hydroperoxyflavin intermediates II-IV and H2O2 release, alphaE238, alphaA75, and alphaA74 are catalytically important active site residues of the alpha-subunit, structure-function relationship | Vibrio harveyi |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
light and dark quantum yield of wild-type and mutant enzymes, overview | Vibrio harveyi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
decanal + FMNH2 + O2 | - |
Vibrio harveyi | decanoate + FMN + H2O + hv | - |
ir |
Subunits | Comment | Organism |
---|---|---|
dimer | alphabeta heterodimer, structure-function relationship, overview | Vibrio harveyi |
Synonyms | Comment | Organism |
---|---|---|
luciferase | - |
Vibrio harveyi |
Vibrio harveyi luciferase | - |
Vibrio harveyi |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
23 | - |
assay at | Vibrio harveyi |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Vibrio harveyi |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMNH2 | - |
Vibrio harveyi |