Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli JM109 (native enzyme) and BL21(DE3) cells (luciferase-mOrange fusion enzyme) | Vibrio harveyi |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00018 | - |
FMNH2 | luciferase-mOrange fusion enzyme, at 22°C, pH not specified in the publication | Vibrio harveyi | |
0.0002 | - |
FMNH2 | recombinant wild type enzyme, at 22°C, pH not specified in the publication | Vibrio harveyi | |
0.00113 | - |
decanal | recombinant wild type enzyme, at 22°C, pH not specified in the publication | Vibrio harveyi | |
0.00165 | - |
decanal | luciferase-mOrange fusion enzyme, at 22°C, pH not specified in the publication | Vibrio harveyi |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vibrio harveyi | - |
- |
- |
Purification (Comment) | Organism |
---|---|
DEAE-Sepharose column chromatography, Superdex 200 gel filtration, and Mono Q column chromatography | Vibrio harveyi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
decanal + FMNH2 + O2 | - |
Vibrio harveyi | decanoic acid + FMN + H2O + hv | - |
? |
Synonyms | Comment | Organism |
---|---|---|
luciferase | - |
Vibrio harveyi |
Lux | - |
Vibrio harveyi |