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Literature summary for 1.14.14.8 extracted from
- Characterization of the anthranilate degradation pathway in Geobacillus thermodenitrificans NG80-2 (2010), Microbiology, 156, 589-595.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Geobacillus thermodenitrificans |
| expression in Escherichia coli | Geobacillus thermodenitrificans |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Geobacillus thermodenitrificans | - |
- |
- |
| Geobacillus thermodenitrificans NG80-2 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| using Ni-NTA chromatography | Geobacillus thermodenitrificans |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 151.3 | - |
substrate anthranilate, pH 9.0, 60°C | Geobacillus thermodenitrificans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-hydroxyphenylacetate + FADH2 + O2 | 67.8% of the activity with anthranilate | Geobacillus thermodenitrificans | ? + FAD + H2O | - |
? |  |
| 2-hydroxyphenylacetate + FADH2 + O2 | 67.8% of the activity with anthranilate | Geobacillus thermodenitrificans NG80-2 | ? + FAD + H2O | - |
? | |
| 2-hydroxyphenylacetate + FADH2 + O2 | 62.79% relative activity | Geobacillus thermodenitrificans | ? | - |
? | |
| 4-hydroxyphenylacetate + FADH2 + O2 | 42.7% of the activity with anthranilate | Geobacillus thermodenitrificans | ? + FAD + H2O | - |
? | |
| 4-hydroxyphenylacetate + FADH2 + O2 | 42.7% of the activity with anthranilate | Geobacillus thermodenitrificans NG80-2 | ? + FAD + H2O | - |
? | |
| 4-hydroxyphenylacetate + FADH2 + O2 | 42.7% relative activity | Geobacillus thermodenitrificans | ? | - |
? | |
| anthranilate + FADH2 + O2 | - |
Geobacillus thermodenitrificans | 3-hydroxyanthranilate + FAD + H2O | - |
? | |
| anthranilate + FADH2 + O2 | - |
Geobacillus thermodenitrificans NG80-2 | 3-hydroxyanthranilate + FAD + H2O | - |
? | |
| anthranilate + FADH2 + O2 | highest activity | Geobacillus thermodenitrificans | 3-hydroxyanthranilate + FAD | - |
? | |
| additional information | no substrate: kynurenine, phenol, 2-nitrobenzylic acid, chlorobenzene, naphthalene, naphthol, toluene or ethylbenzene | Geobacillus thermodenitrificans | ? | - |
? | |
| additional information | no substrate: kynurenine, phenol, 2-nitrobenzylic acid, chlorobenzene, naphthalene, naphthol, toluene or ethylbenzene | Geobacillus thermodenitrificans NG80-2 | ? | - |
? | |
| salicylate + FADH2 + O2 | 49.6% of the activity with anthranilate | Geobacillus thermodenitrificans | ? + FAD + H2O | - |
? | |
| salicylate + FADH2 + O2 | 49.6% of the activity with anthranilate | Geobacillus thermodenitrificans NG80-2 | ? + FAD + H2O | - |
? | |
| salicylate + FADH2 + O2 | 49% relative activity | Geobacillus thermodenitrificans | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| FADH2-utilizing monooxygenase | - |
Geobacillus thermodenitrificans |
| GTNG_3160 | - |
Geobacillus thermodenitrificans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Geobacillus thermodenitrificans |
| 60 | - |
assay at, highest activity | Geobacillus thermodenitrificans |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 70 | - |
Geobacillus thermodenitrificans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
- |
Geobacillus thermodenitrificans |
| 9 | - |
assay at, highest activity | Geobacillus thermodenitrificans |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 11 | - |
Geobacillus thermodenitrificans |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FADH2 | - |
Geobacillus thermodenitrificans | |
| FADH2 | FADH2-utilizing monooxygenase, no reaction with FMNH2 | Geobacillus thermodenitrificans | |
| additional information | no cofactor: FMNH2 | Geobacillus thermodenitrificans |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Geobacillus thermodenitrificans | transcription level increases highly when anthranilate is used as the sole carbon source | up |
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