Cloned (Comment) | Organism |
---|---|
HpaA and HpaH are expressed in Escherichia coli W-21, CC118 and YS1 as well as Klebsiella pneumonia mutant strain AG813 | Klebsiella pneumoniae |
Protein Variants | Comment | Organism |
---|---|---|
additional information | mutant AG813, defective in the 4-hydroxyphenylacetate hydroxylase | Klebsiella pneumoniae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
18680 | - |
x * 58781 flavoprotein HpaA, x * 18680, coupling protein, estimation from gene sequence | Klebsiella pneumoniae |
58781 | - |
x * 58781 flavoprotein HpaA, x * 18680, coupling protein, estimation from gene sequence | Klebsiella pneumoniae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-hydroxyphenylacetate + NADH + O2 | Klebsiella pneumoniae | meta-cleavage pathway | 3,4-dihydroxyphenylacetate + NAD+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Klebsiella pneumoniae | - |
M5a1 strain UN | - |
Purification (Comment) | Organism |
---|---|
partially | Klebsiella pneumoniae |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
4-hydroxyphenylacetate + FADH2 + O2 = 3,4-dihydroxyphenylacetate + FAD + H2O | two protein component enzyme: flavin-containing hydroxylase and coupling protein that appears to be an effector protein, both needed for productive hydroxylation. Enzyme synthesis is induced by 3- or 4-hydroxyphenylacetate | Klebsiella pneumoniae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3,4-dihydroxybenzoate + ? | 30% of 4-hydroxyphenylacetate activity | Klebsiella pneumoniae | ? | - |
? | |
3,4-dihydroxyphenylacetate + ? | 50% of 4-hydroxyphenylacetate activity | Klebsiella pneumoniae | ? | - |
? | |
3-hydroxyphenylacetate + NADH + O2 | - |
Klebsiella pneumoniae | 3,4-hydroxyphenylacetate + NAD+ + H2O | - |
? | |
4-hydroxybenzoate + ? | 15% of 4-hydroxyphenylacetate activity | Klebsiella pneumoniae | 3,4-dihydroxybenzoate + ? | - |
? | |
4-hydroxyphenylacetate + NADH + O2 | - |
Klebsiella pneumoniae | 3,4-dihydroxyphenylacetate + NAD+ + H2O | - |
? | |
4-hydroxyphenylacetate + NADH + O2 | meta-cleavage pathway | Klebsiella pneumoniae | 3,4-dihydroxyphenylacetate + NAD+ + H2O | - |
? | |
catechol + ? | 30% of 4-hydroxyphenylacetate activity | Klebsiella pneumoniae | ? | - |
? | |
L-(3,4-dihydroxy)phenylalanine + ? | 10% of 4-hydroxyphenylacetate | Klebsiella pneumoniae | ? | - |
? | |
L-tyrosine + ? | 8% of 4-hydroxyphenylacetate activity | Klebsiella pneumoniae | L-(3,4-dihydroxy)phenylalanine + ? | - |
? | |
additional information | dihydroxylated compounds are transformed, no reaction with phenylacetate, 4-chlorobenzoate or 2-hydroxyphenylacetate | Klebsiella pneumoniae | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 58781 flavoprotein HpaA, x * 18680, coupling protein, estimation from gene sequence | Klebsiella pneumoniae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | stimulates | Klebsiella pneumoniae | |
NADH | - |
Klebsiella pneumoniae |