Inhibitors | Comment | Organism | Structure |
---|---|---|---|
FAD | inhibitory effect at more than 0.15 mM | Acinetobacter baumannii | |
FMN | inhibitory effect at more than 0.15 mM | Acinetobacter baumannii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.012 | - |
NADH | apparent, with FMN | Acinetobacter baumannii | |
0.014 | - |
4-hydroxyphenylacetate | apparent, with FAD | Acinetobacter baumannii | |
0.019 | - |
4-hydroxyphenylacetate | apparent, with FMN | Acinetobacter baumannii | |
0.028 | - |
NADH | apparent, with FAD | Acinetobacter baumannii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
19000 | - |
2 * 19000 small component, 2 * 59000 large component | Escherichia coli |
30750 | - |
2 * 30750, small component | Pseudomonas putida |
32000 | - |
x * 32000, small component, SDS-PAGE | Acinetobacter baumannii |
38500 | - |
1 * 38500, large component | Pseudomonas putida |
50000 | - |
homotetramer, 4 * 50000, large component, SDS-PAGE | Acinetobacter baumannii |
59000 | - |
2 * 19000 small component, 2 * 59000 large component | Escherichia coli |
73000 | - |
component C1, gel filtration | Acinetobacter baumannii |
209000 | - |
component C2, gel filtration | Acinetobacter baumannii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-hydroxyphenylacetate + NADH + O2 | Escherichia coli | - |
3,4-dihydroxyphenylacetate + NAD+ + H2O | - |
? | |
4-hydroxyphenylacetate + NADH + O2 | Pseudomonas putida | - |
3,4-dihydroxyphenylacetate + NAD+ + H2O | - |
? | |
4-hydroxyphenylacetate + NADH + O2 | Acinetobacter baumannii | biodegradation of aromatic compounds | 3,4-dihydroxyphenylacetate + NAD+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acinetobacter baumannii | - |
- |
- |
Escherichia coli | - |
- |
- |
Pseudomonas putida | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
- |
Pseudomonas putida |
protamine sulfate precipitation, ion-exchange, gel filtration | Acinetobacter baumannii |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
4-hydroxyphenylacetate + FADH2 + O2 = 3,4-dihydroxyphenylacetate + FAD + H2O | different from other hydroxylases. Two-protein component enzyme: component C1 is a flavoprotein and C2 the hydroxylase component. Mechanism: 4-hydroxyphenylacetate binds to C1, then the enzyme-bound flavin is reduced by NADH, this reduced flavin is transferred to C2 and the reoxidation of the flavin occurs concurrently with the hydroxylation of the substrate | Acinetobacter baumannii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
8.89 | - |
C2 component | Acinetobacter baumannii |
201 | - |
C1 component | Acinetobacter baumannii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-hydroxyphenylacetate + NAD(P)H + O2 | 98% of 4-hydroxyphenylacetate activity | Acinetobacter baumannii | 3,4-hydroxyphenylacetate + NAD(P)+ + H2O | - |
? | |
4-hydroxyphenylacetate + NADH + O2 | - |
Escherichia coli | 3,4-dihydroxyphenylacetate + NAD+ + H2O | - |
? | |
4-hydroxyphenylacetate + NADH + O2 | - |
Pseudomonas putida | 3,4-dihydroxyphenylacetate + NAD+ + H2O | - |
? | |
4-hydroxyphenylacetate + NADH + O2 | - |
Acinetobacter baumannii | 3,4-dihydroxyphenylacetate + NAD+ + H2O | - |
? | |
4-hydroxyphenylacetate + NADH + O2 | biodegradation of aromatic compounds | Acinetobacter baumannii | 3,4-dihydroxyphenylacetate + NAD+ + H2O | - |
? | |
additional information | - |
Escherichia coli | ? | - |
? | |
additional information | - |
Pseudomonas putida | ? | - |
? | |
additional information | a variety of aromatic compounds that contain a hydroxyl group in para-position can be hydroxylated. 4-hydroxyphenylacetate hydroxylase is an effector for C1 and substrate for C2 | Acinetobacter baumannii | ? | - |
? | |
phenylacetic acid + ? | - |
Pseudomonas putida | ? | - |
? | |
phenylacetic acid + ? | 97% of 4-hydroxyphenylacetate activity | Acinetobacter baumannii | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 30750, small component | Pseudomonas putida |
dimer | x * 32000, small component, SDS-PAGE | Acinetobacter baumannii |
dimer | 2 * 19000 small component, 2 * 59000 large component | Escherichia coli |
monomer | 1 * 38500, large component | Pseudomonas putida |
tetramer | homotetramer, 4 * 50000, large component, SDS-PAGE | Acinetobacter baumannii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Acinetobacter baumannii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Pseudomonas putida |
7.5 | - |
assay at | Acinetobacter baumannii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Escherichia coli | |
FAD | alternative to FMN for C1 | Acinetobacter baumannii | |
FAD | tightly associated with small component, native cofactor | Pseudomonas putida | |
FADH2 | - |
Escherichia coli | |
FADH2 | - |
Acinetobacter baumannii | |
FMN | - |
Escherichia coli | |
FMN | native cofactor for C1 component | Acinetobacter baumannii | |
FMNH2 | - |
Acinetobacter baumannii | |
NADH | - |
Escherichia coli | |
NADH | - |
Pseudomonas putida | |
NADH | - |
Acinetobacter baumannii | |
NADPH | - |
Escherichia coli | |
riboflavin | - |
Escherichia coli | |
riboflavin | component C1 | Acinetobacter baumannii |