Cloned (Comment) | Organism |
---|---|
the enzyme is CAM plasmid encoded | Pseudomonas putida |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | the enzyme contains [2Fe2 S] cluster and cytochrome P450 | Pseudomonas putida |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(+)-camphor + reduced putidaredoxin + O2 | Pseudomonas putida | - |
(+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O | - |
? | |
(+)-camphor + reduced putidaredoxin + O2 | Pseudomonas putida | under low oxygen conditions borneol is formed instead of 5-ketocamphor | borneol + oxidized putidaredoxin + H2O | - |
? | |
(+)-camphor + reduced putidaredoxin + O2 | Pseudomonas putida ATCC 17453 | - |
(+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O | - |
? | |
(+)-camphor + reduced putidaredoxin + O2 | Pseudomonas putida ATCC 17453 | under low oxygen conditions borneol is formed instead of 5-ketocamphor | borneol + oxidized putidaredoxin + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Pseudomonas putida ATCC 17453 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native C334A enzyme mutant from Pseudomonas putida strain ATCC 17453 by ammonium sulfate fractionation, anion exchange chromatography, and gel filtration | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(+)-camphor + reduced putidaredoxin + O2 | - |
Pseudomonas putida | (+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O | - |
? | |
(+)-camphor + reduced putidaredoxin + O2 | - |
Pseudomonas putida ATCC 17453 | (+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O | - |
? | |
(+)-camphor + reduced putidaredoxin + O2 | under low oxygen conditions borneol is formed instead of 5-ketocamphor | Pseudomonas putida | borneol + oxidized putidaredoxin + H2O | - |
? | |
(+)-camphor + reduced putidaredoxin + O2 | the hydroxylation reaction proceeds via a catalytic cycle in which the reduction of dioxygen is coupled to the oxidation of the substrate. A key intermediate in the catalytic cycle is the iron-oxo species (Fe(IV)=O) | Pseudomonas putida | borneol + oxidized putidaredoxin + H2O | - |
? | |
(+)-camphor + reduced putidaredoxin + O2 | under low oxygen conditions | Pseudomonas putida | borneol + oxidized putidaredoxin + H2O | - |
? | |
(+)-camphor + reduced putidaredoxin + O2 | under low oxygen conditions borneol is formed instead of 5-ketocamphor | Pseudomonas putida ATCC 17453 | borneol + oxidized putidaredoxin + H2O | - |
? | |
(+)-camphor + reduced putidaredoxin + O2 | the hydroxylation reaction proceeds via a catalytic cycle in which the reduction of dioxygen is coupled to the oxidation of the substrate. A key intermediate in the catalytic cycle is the iron-oxo species (Fe(IV)=O) | Pseudomonas putida ATCC 17453 | borneol + oxidized putidaredoxin + H2O | - |
? | |
(+)-camphor + reduced putidaredoxin + O2 | under low oxygen conditions | Pseudomonas putida ATCC 17453 | borneol + oxidized putidaredoxin + H2O | - |
? | |
(+)-exo-5-hydroxycamphor + reduced putidaredoxin + O2 | - |
Pseudomonas putida | 5-oxocamphor + oxidized putidaredoxin + H2O | - |
? | |
additional information | under conditions of low oxygen, Pseudomonas putida cells and the isolated P450cam reduce camphor to borneol, product analysis by GC-MS | Pseudomonas putida | ? | - |
? | |
additional information | under conditions of low oxygen, Pseudomonas putida cells and the isolated P450cam reduce camphor to borneol, product analysis by GC-MS | Pseudomonas putida ATCC 17453 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CYP101 | - |
Pseudomonas putida |
cytochrome p450cam | - |
Pseudomonas putida |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at | Pseudomonas putida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | CYP101 is a heme monoxygenase | Pseudomonas putida | |
putidaredoxin | - |
Pseudomonas putida |
General Information | Comment | Organism |
---|---|---|
additional information | P450cam from Pseudomonas putida oxidizes (1R)-(+)-camphor to 5-exo-hydroxy camphor and further to 5-oxo-camphor | Pseudomonas putida |
physiological function | Pseudomonas putida is capable of detoxification of camphor and borneol, overview | Pseudomonas putida |